Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites
Gray mold disease is caused by Botrytis cinerea and it is the most significant and devastating disease affecting tomato cultivation worldwide. This disease was recently reported infecting tomato fruits in Cameroon Highland, Pahang. Biocontrol agents have been proven to be effective towards many ph...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/17388/ http://journalarticle.ukm.my/17388/1/49_05_10.pdf |
| _version_ | 1848814303043387392 |
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| author | Arafat Hashim, Siti Izera Ismail, Wael Alsultan, Idris Abu Seman, Osamah Rashed, Khairulmazmi Ahmad, |
| author_facet | Arafat Hashim, Siti Izera Ismail, Wael Alsultan, Idris Abu Seman, Osamah Rashed, Khairulmazmi Ahmad, |
| author_sort | Arafat Hashim, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Gray mold disease is caused by Botrytis cinerea and it is the most significant and devastating disease affecting tomato cultivation
worldwide. This disease was recently reported infecting tomato fruits in Cameroon Highland, Pahang. Biocontrol agents
have been proven to be effective towards many phytopathogens. In this study, 43 bacterial isolates were screened against B.
cinerea in-vitro. Five isolates with more than 65% inhibition of radial growth (PIRG) were selected for the subsequent
experiments. Findings in dual culture assay revealed that BM11 and BC4 were the most effective biocontrol agent, with
PIRG of 82.5% and 71.8%, respectively. BM11 and BC4 isolates were identified using the 16S rDNA gene. The identification
results found that BM11 and BC4 were Pseudomonas protegens and Brevibacterium casei, respectively. Ten major bioactive
compounds were successfully identified by Gas chromatography-mass spectrometry (GC-MS) from the culture filtrate of
both biological control agent (BCA) isolates. These bioactive compounds may responsible for antifungal activity. In vivo
study revealed that culture filtrate extract manages to suppress disease lesions on the treated tomato fruit from 0.86 -1.03 cM,
30 days after inoculation. Based on overall findings suggested that P. protegens and B. casei were the promising BCA in
controlling gray mold disease of tomato fruit. |
| first_indexed | 2025-11-15T00:31:56Z |
| format | Article |
| id | oai:generic.eprints.org:17388 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:31:56Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:173882021-08-30T03:54:57Z http://journalarticle.ukm.my/17388/ Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites Arafat Hashim, Siti Izera Ismail, Wael Alsultan, Idris Abu Seman, Osamah Rashed, Khairulmazmi Ahmad, Gray mold disease is caused by Botrytis cinerea and it is the most significant and devastating disease affecting tomato cultivation worldwide. This disease was recently reported infecting tomato fruits in Cameroon Highland, Pahang. Biocontrol agents have been proven to be effective towards many phytopathogens. In this study, 43 bacterial isolates were screened against B. cinerea in-vitro. Five isolates with more than 65% inhibition of radial growth (PIRG) were selected for the subsequent experiments. Findings in dual culture assay revealed that BM11 and BC4 were the most effective biocontrol agent, with PIRG of 82.5% and 71.8%, respectively. BM11 and BC4 isolates were identified using the 16S rDNA gene. The identification results found that BM11 and BC4 were Pseudomonas protegens and Brevibacterium casei, respectively. Ten major bioactive compounds were successfully identified by Gas chromatography-mass spectrometry (GC-MS) from the culture filtrate of both biological control agent (BCA) isolates. These bioactive compounds may responsible for antifungal activity. In vivo study revealed that culture filtrate extract manages to suppress disease lesions on the treated tomato fruit from 0.86 -1.03 cM, 30 days after inoculation. Based on overall findings suggested that P. protegens and B. casei were the promising BCA in controlling gray mold disease of tomato fruit. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17388/1/49_05_10.pdf Arafat Hashim, and Siti Izera Ismail, and Wael Alsultan, and Idris Abu Seman, and Osamah Rashed, and Khairulmazmi Ahmad, (2020) Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites. Malaysian Applied Biology, 49 (5). pp. 89-97. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1078&catid=59:current-view&Itemid=56 |
| spellingShingle | Arafat Hashim, Siti Izera Ismail, Wael Alsultan, Idris Abu Seman, Osamah Rashed, Khairulmazmi Ahmad, Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_full | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_fullStr | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_full_unstemmed | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_short | Control of gray mold disease of tomato caused by Botrytis cinerea using bacterial secondary metabolites |
| title_sort | control of gray mold disease of tomato caused by botrytis cinerea using bacterial secondary metabolites |
| url | http://journalarticle.ukm.my/17388/ http://journalarticle.ukm.my/17388/ http://journalarticle.ukm.my/17388/1/49_05_10.pdf |