Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract
Aquilaria is a multipurpose plant with high commercial and medicinal values. The present study aimed to evaluate the effects of drying methods and extraction solvents on the phenolic and flavonoids content in A. malaccensis (AMA), A. subintegra (ASU), and A. sinensis (ASI). Leaf samples were eithe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/17242/ http://journalarticle.ukm.my/17242/1/49_04_27.pdf |
| _version_ | 1848814264026923008 |
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| author | Wan Ahmad Wan-Nadilah, Dailami Abdul Ghani, Muhammad Syafiq Abd Jalil, Abdul Manaf Ali, Wan Nur Amalina Wan Mamat, Nor Hasima Mahmod, |
| author_facet | Wan Ahmad Wan-Nadilah, Dailami Abdul Ghani, Muhammad Syafiq Abd Jalil, Abdul Manaf Ali, Wan Nur Amalina Wan Mamat, Nor Hasima Mahmod, |
| author_sort | Wan Ahmad Wan-Nadilah, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Aquilaria is a multipurpose plant with high commercial and medicinal values. The present study aimed to evaluate the effects
of drying methods and extraction solvents on the phenolic and flavonoids content in A. malaccensis (AMA), A. subintegra
(ASU), and A. sinensis (ASI). Leaf samples were either Air-Dried (AD) or Oven-Dried (OD) and extracted using 100%
Ethanol (EE100), 70% ethanol (EE70), aqueous (AE), and hexane (HE). There was a significant difference (p<0.05) between
the drying methods and the extracting solvents. The OD in AMA leaf samples gave the highest values of total phenolic
compounds (TPC) (52.98 – 85.15 mg GAE/gm) and total flavonoid compounds (TFC) (2180.97 – 3733.45 QUE ppm). The
highest DPPH radical scavenging was observed in OD-EE70-AMA with the IC50 value of 33.60 µg/mL. Meanwhile,
OD-EE100-AMA gave the lowest IC50 value (0.13 µg/mL) in the α-glucosidase assay, followed by OD-EE70-AMA with
IC50 value 0.69 µg/mL with no significant differences among them. OD-EE70-AMA was found to have a higher content of
TPC and TFC with the most potent DPPH scavenging and α-glucosidase inhibition activities. A strong positive correlation
was reported between bioassays and TPC or TFC, hence indicating that AMA leaf used in this study might have the potential
as a natural antioxidant and an antidiabetic agent. |
| first_indexed | 2025-11-15T00:31:19Z |
| format | Article |
| id | oai:generic.eprints.org:17242 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:31:19Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:172422021-07-29T03:50:57Z http://journalarticle.ukm.my/17242/ Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract Wan Ahmad Wan-Nadilah, Dailami Abdul Ghani, Muhammad Syafiq Abd Jalil, Abdul Manaf Ali, Wan Nur Amalina Wan Mamat, Nor Hasima Mahmod, Aquilaria is a multipurpose plant with high commercial and medicinal values. The present study aimed to evaluate the effects of drying methods and extraction solvents on the phenolic and flavonoids content in A. malaccensis (AMA), A. subintegra (ASU), and A. sinensis (ASI). Leaf samples were either Air-Dried (AD) or Oven-Dried (OD) and extracted using 100% Ethanol (EE100), 70% ethanol (EE70), aqueous (AE), and hexane (HE). There was a significant difference (p<0.05) between the drying methods and the extracting solvents. The OD in AMA leaf samples gave the highest values of total phenolic compounds (TPC) (52.98 – 85.15 mg GAE/gm) and total flavonoid compounds (TFC) (2180.97 – 3733.45 QUE ppm). The highest DPPH radical scavenging was observed in OD-EE70-AMA with the IC50 value of 33.60 µg/mL. Meanwhile, OD-EE100-AMA gave the lowest IC50 value (0.13 µg/mL) in the α-glucosidase assay, followed by OD-EE70-AMA with IC50 value 0.69 µg/mL with no significant differences among them. OD-EE70-AMA was found to have a higher content of TPC and TFC with the most potent DPPH scavenging and α-glucosidase inhibition activities. A strong positive correlation was reported between bioassays and TPC or TFC, hence indicating that AMA leaf used in this study might have the potential as a natural antioxidant and an antidiabetic agent. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17242/1/49_04_27.pdf Wan Ahmad Wan-Nadilah, and Dailami Abdul Ghani, and Muhammad Syafiq Abd Jalil, and Abdul Manaf Ali, and Wan Nur Amalina Wan Mamat, and Nor Hasima Mahmod, (2020) Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract. Malaysian Applied Biology, 49 (4). pp. 201-205. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
| spellingShingle | Wan Ahmad Wan-Nadilah, Dailami Abdul Ghani, Muhammad Syafiq Abd Jalil, Abdul Manaf Ali, Wan Nur Amalina Wan Mamat, Nor Hasima Mahmod, Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract |
| title | Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract |
| title_full | Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract |
| title_fullStr | Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract |
| title_full_unstemmed | Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract |
| title_short | Effects of drying methods and solvents on phenolic and flavonoids content, DPPH radical scavenging and alpha-glucosidase activities of Aquilaria leaves extract |
| title_sort | effects of drying methods and solvents on phenolic and flavonoids content, dpph radical scavenging and alpha-glucosidase activities of aquilaria leaves extract |
| url | http://journalarticle.ukm.my/17242/ http://journalarticle.ukm.my/17242/ http://journalarticle.ukm.my/17242/1/49_04_27.pdf |