Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11
The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB) with a specific 4HB molar fraction is still limited. In t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/17225/ http://journalarticle.ukm.my/17225/1/49_04_10.pdf |
| _version_ | 1848814259461423104 |
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| author | Jun, Meng Chai Shaliny Hari Rama Krishnan, Tan, Vincent Yee Hang Hanis Athirah Mohd Hamdan, Nurul Nadhirah Ruzelan, Sevakumaran Vigneswari, |
| author_facet | Jun, Meng Chai Shaliny Hari Rama Krishnan, Tan, Vincent Yee Hang Hanis Athirah Mohd Hamdan, Nurul Nadhirah Ruzelan, Sevakumaran Vigneswari, |
| author_sort | Jun, Meng Chai |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well
as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB)
with a specific 4HB molar fraction is still limited. In this study, carbon precursors at different combinations and concentration
ratios have been used in the production of P(3HB-co-4HB) bioplastics using locally isolated Cupriavidus sp. TMT11. The
carbon precursors 1,6-hexandiol, 1,4-butanediol, and γ-butyrolactone were used to screen for high cell biomass, PHA content,
and 4HB molar fraction through Gas Chromatography (GC) analysis. Generally, the combination of various carbon precursors
showed an increase in cell biomass. The carbon combination of 1,4-butanediol and γ-butyrolactone at the ratio of 4:2 and
carbon combination of 1,6-hexanediol and γ-butyrolactone at a ratio of 1:5 showed high amount of cell biomass above 0.35
g/L on day 3. Nevertheless, the 4HB molar fraction of both the combination was recorded as 9±0.27 mol% and 14±1.1
mol% respectively. The lowest amount of cell biomass and PHA yield were recorded with the carbon combination of
1,6-hexanediol and 1,4-butanediol with the ratio 5:1 at 0.55±0.13 g/L. However, the highest 4HB molar fraction of 89.37±3.6
mol% 4HB was recorded with this combination. The 4HB molar fraction above 80 mol% was reported with a carbon
combination of 1,6-hexanediol +1,4-butanediol at 5:1, 1:1, and 2:4 ratio. The varying combination of carbon precursors
biosynthesized a wide range of 4HB molar fractions ranging from 9.07 to 89.37 mol% 4HB. |
| first_indexed | 2025-11-15T00:31:14Z |
| format | Article |
| id | oai:generic.eprints.org:17225 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:31:14Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:172252021-07-29T03:03:26Z http://journalarticle.ukm.my/17225/ Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 Jun, Meng Chai Shaliny Hari Rama Krishnan, Tan, Vincent Yee Hang Hanis Athirah Mohd Hamdan, Nurul Nadhirah Ruzelan, Sevakumaran Vigneswari, The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB) with a specific 4HB molar fraction is still limited. In this study, carbon precursors at different combinations and concentration ratios have been used in the production of P(3HB-co-4HB) bioplastics using locally isolated Cupriavidus sp. TMT11. The carbon precursors 1,6-hexandiol, 1,4-butanediol, and γ-butyrolactone were used to screen for high cell biomass, PHA content, and 4HB molar fraction through Gas Chromatography (GC) analysis. Generally, the combination of various carbon precursors showed an increase in cell biomass. The carbon combination of 1,4-butanediol and γ-butyrolactone at the ratio of 4:2 and carbon combination of 1,6-hexanediol and γ-butyrolactone at a ratio of 1:5 showed high amount of cell biomass above 0.35 g/L on day 3. Nevertheless, the 4HB molar fraction of both the combination was recorded as 9±0.27 mol% and 14±1.1 mol% respectively. The lowest amount of cell biomass and PHA yield were recorded with the carbon combination of 1,6-hexanediol and 1,4-butanediol with the ratio 5:1 at 0.55±0.13 g/L. However, the highest 4HB molar fraction of 89.37±3.6 mol% 4HB was recorded with this combination. The 4HB molar fraction above 80 mol% was reported with a carbon combination of 1,6-hexanediol +1,4-butanediol at 5:1, 1:1, and 2:4 ratio. The varying combination of carbon precursors biosynthesized a wide range of 4HB molar fractions ranging from 9.07 to 89.37 mol% 4HB. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17225/1/49_04_10.pdf Jun, Meng Chai and Shaliny Hari Rama Krishnan, and Tan, Vincent Yee Hang and Hanis Athirah Mohd Hamdan, and Nurul Nadhirah Ruzelan, and Sevakumaran Vigneswari, (2020) Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11. Malaysian Applied Biology, 49 (4). pp. 79-84. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
| spellingShingle | Jun, Meng Chai Shaliny Hari Rama Krishnan, Tan, Vincent Yee Hang Hanis Athirah Mohd Hamdan, Nurul Nadhirah Ruzelan, Sevakumaran Vigneswari, Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 |
| title | Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 |
| title_full | Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 |
| title_fullStr | Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 |
| title_full_unstemmed | Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 |
| title_short | Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 |
| title_sort | effects of various carbon precursors combination in regulating the molar fraction of p(3hb-co-4hb) using locally isolated cupriavidus sp. tmt11 |
| url | http://journalarticle.ukm.my/17225/ http://journalarticle.ukm.my/17225/ http://journalarticle.ukm.my/17225/1/49_04_10.pdf |