Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent

Azo dyes are widely used in the textile industry. The release of these undesirable dye effluents to the environment can be toxic and carcinogenic towards humans and other organisms. The removal of these dyestuff wastes using the biodegradation method is to consider eco-friendlier compared to physi...

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Main Authors: Badrul Khalid Ariffin, Fazilah Ariffin
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/17216/
http://journalarticle.ukm.my/17216/1/49_04_01.pdf
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author Badrul Khalid Ariffin,
Fazilah Ariffin,
author_facet Badrul Khalid Ariffin,
Fazilah Ariffin,
author_sort Badrul Khalid Ariffin,
building UKM Institutional Repository
collection Online Access
description Azo dyes are widely used in the textile industry. The release of these undesirable dye effluents to the environment can be toxic and carcinogenic towards humans and other organisms. The removal of these dyestuff wastes using the biodegradation method is to consider eco-friendlier compared to physical and chemical methods. Thus, this study was conducted to identify and analyze the biodegradation activity of the azo dye by the potential bacteria isolated from textile dye effluent. For decolorization analysis, the isolated bacteria were inoculated into flasks containing mineral salt medium added with Reactive Green 19 dye (50 mg/L) and was incubated for 9 days at 37°C. Spectrophotometry analysis was carried out at 635 nm to analyze the level of azo dye degradation. From the analysis, we found that the optimum pH, temperature, and dye concentration for the decolorization were detected at pH 7, 37°C, and 50 mg/L, respectively. The identified bacteria Pseudomonas aeruginosa showed 94% decolorization of Reactive Green 19 dye after 9 days of incubation. The result of decolorization activity by this bacterial strain can be used in the biological treatment of textile effluent.
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spelling oai:generic.eprints.org:172162021-07-29T02:43:42Z http://journalarticle.ukm.my/17216/ Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent Badrul Khalid Ariffin, Fazilah Ariffin, Azo dyes are widely used in the textile industry. The release of these undesirable dye effluents to the environment can be toxic and carcinogenic towards humans and other organisms. The removal of these dyestuff wastes using the biodegradation method is to consider eco-friendlier compared to physical and chemical methods. Thus, this study was conducted to identify and analyze the biodegradation activity of the azo dye by the potential bacteria isolated from textile dye effluent. For decolorization analysis, the isolated bacteria were inoculated into flasks containing mineral salt medium added with Reactive Green 19 dye (50 mg/L) and was incubated for 9 days at 37°C. Spectrophotometry analysis was carried out at 635 nm to analyze the level of azo dye degradation. From the analysis, we found that the optimum pH, temperature, and dye concentration for the decolorization were detected at pH 7, 37°C, and 50 mg/L, respectively. The identified bacteria Pseudomonas aeruginosa showed 94% decolorization of Reactive Green 19 dye after 9 days of incubation. The result of decolorization activity by this bacterial strain can be used in the biological treatment of textile effluent. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17216/1/49_04_01.pdf Badrul Khalid Ariffin, and Fazilah Ariffin, (2020) Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent. Malaysian Applied Biology, 49 (4). pp. 1-8. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56
spellingShingle Badrul Khalid Ariffin,
Fazilah Ariffin,
Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent
title Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent
title_full Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent
title_fullStr Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent
title_full_unstemmed Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent
title_short Biodegradation of azo dye (Reactive Green 19) by Pseudomonas aeruginosa isolated from textile effluent
title_sort biodegradation of azo dye (reactive green 19) by pseudomonas aeruginosa isolated from textile effluent
url http://journalarticle.ukm.my/17216/
http://journalarticle.ukm.my/17216/
http://journalarticle.ukm.my/17216/1/49_04_01.pdf