Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans
Vitamin E is an established antioxidant. However, the effect of vitamin E on healthspan, which deteriorates during ageing, has not been determined because most related studies have emphasized its effects on lifespan. Therefore, the purpose of this study was to determine the effect of palm tocotrieno...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/16501/ http://journalarticle.ukm.my/16501/1/14.pdf |
| _version_ | 1848814068445478912 |
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| author | Tan, Tzer Sien Muhammad Firdaus Sathik Rahman, Siti Sara Ismail, Nur Najihah Mohamad, Ahmad Hazim Mustaffa, Geetha Gunasekaran, Siti Bazilah Zulkefli, Goon, Jo Aan |
| author_facet | Tan, Tzer Sien Muhammad Firdaus Sathik Rahman, Siti Sara Ismail, Nur Najihah Mohamad, Ahmad Hazim Mustaffa, Geetha Gunasekaran, Siti Bazilah Zulkefli, Goon, Jo Aan |
| author_sort | Tan, Tzer Sien |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Vitamin E is an established antioxidant. However, the effect of vitamin E on healthspan, which deteriorates during ageing, has not been determined because most related studies have emphasized its effects on lifespan. Therefore, the purpose of this study was to determine the effect of palm tocotrienol-rich fraction (TRF) on the lifespan, locomotion and thermotolerance of Caenorhabditis elegans, which share many common gene sequences with humans. The nematodes were treated with different concentrations of TRF (0 - 200 μg/mL), and the number of surviving nematodes at each concentration (N=30, duplicate) was counted daily under a light microscope to determine the optimal dose of treatment. The nematodes were divided into 3 groups, namely; control, Tween-80 (vehicle) and TRF-treated. Locomotion and thermotolerance were determined on day 4 and 12 of treatment in adult nematodes. ImageJ was used for locomotion analysis, and thermotolerance was determined based on nematode survivals after exposure to 37 °C. TRF-treated C. elegans had significantly longer lifespans compared to controls (P = 0.003). The TRF group (50 μg/mL) had the longest mean lifespan (23.5 days), which was significantly longer compared to controls, (18.5 days; (P = 0.002). However, locomotion was similar between all groups. In the thermotolerance assay, the survival determined on day 4 and day 12 of TRF-treatment was higher compared to controls (P= 0.046). Interestingly, the Tween- 80-treated group showed similar results as the TRF-treated group compared to controls. The findings indicate that TRF prolongs the lifespan and increases the thermotolerance of C. elegans but does not improve the locomotion of the worms as they age. |
| first_indexed | 2025-11-15T00:28:12Z |
| format | Article |
| id | oai:generic.eprints.org:16501 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:28:12Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:165012021-04-28T07:02:30Z http://journalarticle.ukm.my/16501/ Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans Tan, Tzer Sien Muhammad Firdaus Sathik Rahman, Siti Sara Ismail, Nur Najihah Mohamad, Ahmad Hazim Mustaffa, Geetha Gunasekaran, Siti Bazilah Zulkefli, Goon, Jo Aan Vitamin E is an established antioxidant. However, the effect of vitamin E on healthspan, which deteriorates during ageing, has not been determined because most related studies have emphasized its effects on lifespan. Therefore, the purpose of this study was to determine the effect of palm tocotrienol-rich fraction (TRF) on the lifespan, locomotion and thermotolerance of Caenorhabditis elegans, which share many common gene sequences with humans. The nematodes were treated with different concentrations of TRF (0 - 200 μg/mL), and the number of surviving nematodes at each concentration (N=30, duplicate) was counted daily under a light microscope to determine the optimal dose of treatment. The nematodes were divided into 3 groups, namely; control, Tween-80 (vehicle) and TRF-treated. Locomotion and thermotolerance were determined on day 4 and 12 of treatment in adult nematodes. ImageJ was used for locomotion analysis, and thermotolerance was determined based on nematode survivals after exposure to 37 °C. TRF-treated C. elegans had significantly longer lifespans compared to controls (P = 0.003). The TRF group (50 μg/mL) had the longest mean lifespan (23.5 days), which was significantly longer compared to controls, (18.5 days; (P = 0.002). However, locomotion was similar between all groups. In the thermotolerance assay, the survival determined on day 4 and day 12 of TRF-treatment was higher compared to controls (P= 0.046). Interestingly, the Tween- 80-treated group showed similar results as the TRF-treated group compared to controls. The findings indicate that TRF prolongs the lifespan and increases the thermotolerance of C. elegans but does not improve the locomotion of the worms as they age. Penerbit Universiti Kebangsaan Malaysia 2021-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16501/1/14.pdf Tan, Tzer Sien and Muhammad Firdaus Sathik Rahman, and Siti Sara Ismail, and Nur Najihah Mohamad, and Ahmad Hazim Mustaffa, and Geetha Gunasekaran, and Siti Bazilah Zulkefli, and Goon, Jo Aan (2021) Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans. Sains Malaysiana, 50 (2). pp. 429-436. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil2_2021/KandunganJilid50Bil2_2021.html |
| spellingShingle | Tan, Tzer Sien Muhammad Firdaus Sathik Rahman, Siti Sara Ismail, Nur Najihah Mohamad, Ahmad Hazim Mustaffa, Geetha Gunasekaran, Siti Bazilah Zulkefli, Goon, Jo Aan Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans |
| title | Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans |
| title_full | Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans |
| title_fullStr | Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans |
| title_full_unstemmed | Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans |
| title_short | Effect of the tocotrienol-rich fraction (TRF) on the healthspan of Caenorhabditis elegans |
| title_sort | effect of the tocotrienol-rich fraction (trf) on the healthspan of caenorhabditis elegans |
| url | http://journalarticle.ukm.my/16501/ http://journalarticle.ukm.my/16501/ http://journalarticle.ukm.my/16501/1/14.pdf |