The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer
Mangrove plants produce polyisoprenoid alcohol. The polyisoprenoid consists of polyprenol and dolichol, which in pharmacological activity act as anticancer agents. The major polyisoprenoid compound of mangrove plants Ceriops tagal and Rhizophora mucronata was reported as dolichol. The present st...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
|
| Online Access: | http://journalarticle.ukm.my/16403/ http://journalarticle.ukm.my/16403/1/18.pdf |
| _version_ | 1848814043253440512 |
|---|---|
| author | Istiqomah, Meighina Atika Hasibuan, Poppy Anjelisa Zaitun Nuryawan, Arif Sumaiyah Sumaiyah, Siregar, Etti Sartina Mohammad Basyuni, |
| author_facet | Istiqomah, Meighina Atika Hasibuan, Poppy Anjelisa Zaitun Nuryawan, Arif Sumaiyah Sumaiyah, Siregar, Etti Sartina Mohammad Basyuni, |
| author_sort | Istiqomah, Meighina Atika |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Mangrove plants produce polyisoprenoid alcohol. The polyisoprenoid consists of polyprenol and dolichol, which in
pharmacological activity act as anticancer agents. The major polyisoprenoid compound of mangrove plants Ceriops
tagal and Rhizophora mucronata was reported as dolichol. The present study was conducted to examine the anticancer
effects of dolichol from C. tagal and R. mucronata leaves on WiDr cells and cell cycle-related cancer for 24 h and to
evaluate the regulation of five genes, p53, EGFR, PI3K, Akt, and mTOR. The inhibited cell cycle was analysed by flow
cytometry and the gene expression of p53, EGFR, PI3K, Akt, and mTOR was determined using reverse transcription�polymerase chain reaction (RT-PCR) method. Dolichol from C. tagal was more effective than that from R. mucronata,
where it worked on the G0/G1 cycle for 87.94% and 82.36%, respectively, and regulated positive control 5-FU on
the G0/G1 cycle (88.12%), S (9.52%) and G2-M (6.42%). The upregulation (p53) and downregulation (EGFR)
contributed to the contracting cell cycle of colon cancer cells (WiDr) in PI3K, Akt and mTOR genes. To summarise, the
current study suggests significant pharmacological properties of dolichols in C. tagal and R. mucronata leaves, which
worked explicitly in the G0/G1 phase. |
| first_indexed | 2025-11-15T00:27:48Z |
| format | Article |
| id | oai:generic.eprints.org:16403 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:27:48Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:164032021-04-13T04:18:58Z http://journalarticle.ukm.my/16403/ The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer Istiqomah, Meighina Atika Hasibuan, Poppy Anjelisa Zaitun Nuryawan, Arif Sumaiyah Sumaiyah, Siregar, Etti Sartina Mohammad Basyuni, Mangrove plants produce polyisoprenoid alcohol. The polyisoprenoid consists of polyprenol and dolichol, which in pharmacological activity act as anticancer agents. The major polyisoprenoid compound of mangrove plants Ceriops tagal and Rhizophora mucronata was reported as dolichol. The present study was conducted to examine the anticancer effects of dolichol from C. tagal and R. mucronata leaves on WiDr cells and cell cycle-related cancer for 24 h and to evaluate the regulation of five genes, p53, EGFR, PI3K, Akt, and mTOR. The inhibited cell cycle was analysed by flow cytometry and the gene expression of p53, EGFR, PI3K, Akt, and mTOR was determined using reverse transcription�polymerase chain reaction (RT-PCR) method. Dolichol from C. tagal was more effective than that from R. mucronata, where it worked on the G0/G1 cycle for 87.94% and 82.36%, respectively, and regulated positive control 5-FU on the G0/G1 cycle (88.12%), S (9.52%) and G2-M (6.42%). The upregulation (p53) and downregulation (EGFR) contributed to the contracting cell cycle of colon cancer cells (WiDr) in PI3K, Akt and mTOR genes. To summarise, the current study suggests significant pharmacological properties of dolichols in C. tagal and R. mucronata leaves, which worked explicitly in the G0/G1 phase. Penerbit Universiti Kebangsaan Malaysia 2021-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16403/1/18.pdf Istiqomah, Meighina Atika and Hasibuan, Poppy Anjelisa Zaitun and Nuryawan, Arif and Sumaiyah Sumaiyah, and Siregar, Etti Sartina and Mohammad Basyuni, (2021) The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer. Sains Malaysiana, 50 (1). pp. 181-189. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil1_2021/KandunganJilid50Bil1_2021.html |
| spellingShingle | Istiqomah, Meighina Atika Hasibuan, Poppy Anjelisa Zaitun Nuryawan, Arif Sumaiyah Sumaiyah, Siregar, Etti Sartina Mohammad Basyuni, The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer |
| title | The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer |
| title_full | The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer |
| title_fullStr | The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer |
| title_full_unstemmed | The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer |
| title_short | The anticancer compound dolichol from Ceriops tagal and Rhizophora mucronata leaves regulates gene expressions in WiDr colon cancer |
| title_sort | anticancer compound dolichol from ceriops tagal and rhizophora mucronata leaves regulates gene expressions in widr colon cancer |
| url | http://journalarticle.ukm.my/16403/ http://journalarticle.ukm.my/16403/ http://journalarticle.ukm.my/16403/1/18.pdf |