Genetic characterization of the Central Javanese duck using microsatellite markers
Duck conservation strategy based on genetic characteristics has been undertaken to preserve indigenous breeds and produce high quality offspring. In addition, the genetic characteristic analysis provides further information for studying avian influenza viruses. The aim of this research was to st...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/16391/ http://journalarticle.ukm.my/16391/1/6.pdf |
| _version_ | 1848814040140218368 |
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| author | R, Susanti Ari, Yuniastuti Agustin, Dian Kartikasari |
| author_facet | R, Susanti Ari, Yuniastuti Agustin, Dian Kartikasari |
| author_sort | R, Susanti |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Duck conservation strategy based on genetic characteristics has been undertaken to preserve indigenous breeds
and produce high quality offspring. In addition, the genetic characteristic analysis provides further information for
studying avian influenza viruses. The aim of this research was to study the genetic characterisation of indigenous
Central Javanese duck in Indonesia using microsatellite markers. Microsatellite or simple sequence repeats (SSR) or
short tandem repeats (STR) of various breeds of Central Javanese duck were analysed on 35 local ducks which
consisted of seven variants of Javanese ducks in Central Java. Ten pairs of duck microsatellite primers were used to
amplify the DNA from feathers. Nine out of 10 microsatellite loci (90%) demonstrated polymorphic patterns containing
50 alleles. The number of alelles varied from two to 10 alleles at various polymorphic microsatellite loci with an
average of 5.56 alelles per locus. From the study, CAUD013 locus was determined as the highest heterozygosity
with 0.882 score while CAUD030 locus was the lowest one with 0.034 score. Similarly, CAUD030 locus had the
lowest PIC score (0.30) whereas the CAUD013 locus had the highest PIC value (0.869). Hence, it was showed that
microsatellite markers were highly polymorphic in several loci for the Central Javanese duck and may be useful for
genetic description of other native duck in terms of species conservation. |
| first_indexed | 2025-11-15T00:27:45Z |
| format | Article |
| id | oai:generic.eprints.org:16391 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:27:45Z |
| publishDate | 2021 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:163912021-04-13T03:10:26Z http://journalarticle.ukm.my/16391/ Genetic characterization of the Central Javanese duck using microsatellite markers R, Susanti Ari, Yuniastuti Agustin, Dian Kartikasari Duck conservation strategy based on genetic characteristics has been undertaken to preserve indigenous breeds and produce high quality offspring. In addition, the genetic characteristic analysis provides further information for studying avian influenza viruses. The aim of this research was to study the genetic characterisation of indigenous Central Javanese duck in Indonesia using microsatellite markers. Microsatellite or simple sequence repeats (SSR) or short tandem repeats (STR) of various breeds of Central Javanese duck were analysed on 35 local ducks which consisted of seven variants of Javanese ducks in Central Java. Ten pairs of duck microsatellite primers were used to amplify the DNA from feathers. Nine out of 10 microsatellite loci (90%) demonstrated polymorphic patterns containing 50 alleles. The number of alelles varied from two to 10 alleles at various polymorphic microsatellite loci with an average of 5.56 alelles per locus. From the study, CAUD013 locus was determined as the highest heterozygosity with 0.882 score while CAUD030 locus was the lowest one with 0.034 score. Similarly, CAUD030 locus had the lowest PIC score (0.30) whereas the CAUD013 locus had the highest PIC value (0.869). Hence, it was showed that microsatellite markers were highly polymorphic in several loci for the Central Javanese duck and may be useful for genetic description of other native duck in terms of species conservation. Penerbit Universiti Kebangsaan Malaysia 2021-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16391/1/6.pdf R, Susanti and Ari, Yuniastuti and Agustin, Dian Kartikasari (2021) Genetic characterization of the Central Javanese duck using microsatellite markers. Sains Malaysiana, 50 (1). pp. 53-61. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil1_2021/KandunganJilid50Bil1_2021.html |
| spellingShingle | R, Susanti Ari, Yuniastuti Agustin, Dian Kartikasari Genetic characterization of the Central Javanese duck using microsatellite markers |
| title | Genetic characterization of the Central Javanese duck using microsatellite markers |
| title_full | Genetic characterization of the Central Javanese duck using microsatellite markers |
| title_fullStr | Genetic characterization of the Central Javanese duck using microsatellite markers |
| title_full_unstemmed | Genetic characterization of the Central Javanese duck using microsatellite markers |
| title_short | Genetic characterization of the Central Javanese duck using microsatellite markers |
| title_sort | genetic characterization of the central javanese duck using microsatellite markers |
| url | http://journalarticle.ukm.my/16391/ http://journalarticle.ukm.my/16391/ http://journalarticle.ukm.my/16391/1/6.pdf |