Isolation and characterization of pisang tongkat langit (Musa troglodytarum L.) ACS1 and ACO1 gene expression during fruit ripening process
Pisang tongkat langit (Musa troglodytarum L.) is a banana cultivar originated from Eastern Indonesia. Previous studies showed that ACS1 and ACO1 are genes that play an important role in banana ethylene biosynthesis. However, characterization and analysis of M. troglodytarum ACS1 and ACO1 genes hav...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16029/ http://journalarticle.ukm.my/16029/1/49_02_13.pdf |
| Summary: | Pisang tongkat langit (Musa troglodytarum L.) is a banana cultivar originated from Eastern Indonesia. Previous studies
showed that ACS1 and ACO1 are genes that play an important role in banana ethylene biosynthesis. However, characterization
and analysis of M. troglodytarum ACS1 and ACO1 genes have not been reported. This study aims to isolate and characterize
the ACS1 and ACO1 gene expression profile during fruit ripening process. Gene fragment isolation was performed using
PCR based method. Results showed that the isolated genomic fragments of ACS1 was 400 bp and ACO1 was 500 bp. cDNA
fragments of ACS1 was 200 bp and ACO1 was 300 bp. Analysis of genomic fragments using BLAST NCBI showed that
ACS1 had a high homology with 1818 bp ACS1 gene of Musa acuminata AAA Group (GenBank: GQ396304.1), while
ACO1 gene had the highest homology with 1593 bp ACO1 gene of M. acuminata AAA Group (GenBank: AJ223232.1).
ACS1 genomic fragments of M. troglodytarum had one intron and two exons, while ACO1 had two introns and three exons.
Homology analysis showed that gene fragment of ACS1 and ACO1 of M. troglodytarum had conserved region similar to
M. acuminata. |
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