Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18
The protease from Chromohalobacter japonicus BK-AB18 was produced by growing bacteria in a LB medium containing 5% casein and 5% NaCl. The crude protease was partially purified by three levels of ammonium sulfate concentration (ranges of 0–70%, 70–75% and 75–80%) and the highest specific activity...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16022/ http://journalarticle.ukm.my/16022/1/49_02_06.pdf |
| _version_ | 1848813945078415360 |
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| author | Achmad, Dwi Isyana Ihsanawati, Hertadi, Rukman |
| author_facet | Achmad, Dwi Isyana Ihsanawati, Hertadi, Rukman |
| author_sort | Achmad, Dwi Isyana |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The protease from Chromohalobacter japonicus BK-AB18 was produced by growing bacteria in a LB medium containing
5% casein and 5% NaCl. The crude protease was partially purified by three levels of ammonium sulfate concentration (ranges
of 0–70%, 70–75% and 75–80%) and the highest specific activity was exhibited in the range of 75–80%. The enzyme has a
relative molecular weight of 65 kDa. The protease in this fraction had the highest activity in the following optimum conditions:
7.5% NaCl, a pH of 9.0 and a temperature of 45°C. The activity of the enzyme at the optimum pH and temperature was
enhanced by the addition of a Ca2+ ion, but its activity was significantly inhibited by EDTA, hence this enzyme is included as
metalloenzyme. Interestingly, the protease activity increased when exposed to a concentration of 0.01% and 0.05% SDS, and
was relatively stable in this solution up to a concentration of 10%. It is thus demonstrated that C. japonicus BK-AB18 is a
potential source to produce extracellular protease that can be applied in the surfactant/detergent industry. |
| first_indexed | 2025-11-15T00:26:15Z |
| format | Article |
| id | oai:generic.eprints.org:16022 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:26:15Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:160222020-12-18T02:14:10Z http://journalarticle.ukm.my/16022/ Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18 Achmad, Dwi Isyana Ihsanawati, Hertadi, Rukman The protease from Chromohalobacter japonicus BK-AB18 was produced by growing bacteria in a LB medium containing 5% casein and 5% NaCl. The crude protease was partially purified by three levels of ammonium sulfate concentration (ranges of 0–70%, 70–75% and 75–80%) and the highest specific activity was exhibited in the range of 75–80%. The enzyme has a relative molecular weight of 65 kDa. The protease in this fraction had the highest activity in the following optimum conditions: 7.5% NaCl, a pH of 9.0 and a temperature of 45°C. The activity of the enzyme at the optimum pH and temperature was enhanced by the addition of a Ca2+ ion, but its activity was significantly inhibited by EDTA, hence this enzyme is included as metalloenzyme. Interestingly, the protease activity increased when exposed to a concentration of 0.01% and 0.05% SDS, and was relatively stable in this solution up to a concentration of 10%. It is thus demonstrated that C. japonicus BK-AB18 is a potential source to produce extracellular protease that can be applied in the surfactant/detergent industry. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16022/1/49_02_06.pdf Achmad, Dwi Isyana and Ihsanawati, and Hertadi, Rukman (2020) Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18. Malaysian Applied Biology, 49 (2). pp. 37-42. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1003&catid=59:current-view&Itemid=56 |
| spellingShingle | Achmad, Dwi Isyana Ihsanawati, Hertadi, Rukman Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18 |
| title | Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18 |
| title_full | Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18 |
| title_fullStr | Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18 |
| title_full_unstemmed | Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18 |
| title_short | Isolation and characterization of a surfactant-stable protease from halophilic bacteria Chromohalobacter Japonicus BK-AB18 |
| title_sort | isolation and characterization of a surfactant-stable protease from halophilic bacteria chromohalobacter japonicus bk-ab18 |
| url | http://journalarticle.ukm.my/16022/ http://journalarticle.ukm.my/16022/ http://journalarticle.ukm.my/16022/1/49_02_06.pdf |