Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi
Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the production of thermostable lipase. In this study, the l...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16019/ http://journalarticle.ukm.my/16019/1/49_02_03.pdf |
| _version_ | 1848813944242700288 |
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| author | Khalilan Lambangsari, Moeis, Maelita Ramdani Elvi Restiawaty, |
| author_facet | Khalilan Lambangsari, Moeis, Maelita Ramdani Elvi Restiawaty, |
| author_sort | Khalilan Lambangsari, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North
Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the
production of thermostable lipase. In this study, the lipase gene from the selected isolate was cloned, sequenced, and in silico
analysis was performed to predict the characteristics of the lipase protein. One of three isolates examined, showed a positive
reaction. Phylogenetic analysis of 16S rRNA gene showed that the isolate was a novel bacteria closely related to Bacillus
safensis strain FO-26b with 96,87% homology. Amino acid sequence analysis of the lipase showed similarity between lipase
of Bacillus pumilus strain B106 with 98% homology. Further analysis at the protein level indicated that the lipase isolated
from marine water was a member of family I.4 of ‘true lipase’ group. |
| first_indexed | 2025-11-15T00:26:14Z |
| format | Article |
| id | oai:generic.eprints.org:16019 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:26:14Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:160192020-12-18T02:08:49Z http://journalarticle.ukm.my/16019/ Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi Khalilan Lambangsari, Moeis, Maelita Ramdani Elvi Restiawaty, Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the production of thermostable lipase. In this study, the lipase gene from the selected isolate was cloned, sequenced, and in silico analysis was performed to predict the characteristics of the lipase protein. One of three isolates examined, showed a positive reaction. Phylogenetic analysis of 16S rRNA gene showed that the isolate was a novel bacteria closely related to Bacillus safensis strain FO-26b with 96,87% homology. Amino acid sequence analysis of the lipase showed similarity between lipase of Bacillus pumilus strain B106 with 98% homology. Further analysis at the protein level indicated that the lipase isolated from marine water was a member of family I.4 of ‘true lipase’ group. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16019/1/49_02_03.pdf Khalilan Lambangsari, and Moeis, Maelita Ramdani and Elvi Restiawaty, (2020) Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi. Malaysian Applied Biology, 49 (2). pp. 13-17. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1003&catid=59:current-view&Itemid=56 |
| spellingShingle | Khalilan Lambangsari, Moeis, Maelita Ramdani Elvi Restiawaty, Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi |
| title | Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi |
| title_full | Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi |
| title_fullStr | Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi |
| title_full_unstemmed | Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi |
| title_short | Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi |
| title_sort | cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of kawio, north sulawesi |
| url | http://journalarticle.ukm.my/16019/ http://journalarticle.ukm.my/16019/ http://journalarticle.ukm.my/16019/1/49_02_03.pdf |