Cloning and in silico studies of lipase gene of a thermophilic bacteria isolated from the hydrothermal vent area of Kawio, North Sulawesi
Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the production of thermostable lipase. In this study, the l...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16019/ http://journalarticle.ukm.my/16019/1/49_02_03.pdf |
| Summary: | Underwater volcano is an example of a natural habitat of thermophilic bacteria, such as hydrothermal vent Kawio in North
Sulawesi. The focus of this research was to screen the bacterial isolates originally from hydrothermal vent Kawio for the
production of thermostable lipase. In this study, the lipase gene from the selected isolate was cloned, sequenced, and in silico
analysis was performed to predict the characteristics of the lipase protein. One of three isolates examined, showed a positive
reaction. Phylogenetic analysis of 16S rRNA gene showed that the isolate was a novel bacteria closely related to Bacillus
safensis strain FO-26b with 96,87% homology. Amino acid sequence analysis of the lipase showed similarity between lipase
of Bacillus pumilus strain B106 with 98% homology. Further analysis at the protein level indicated that the lipase isolated
from marine water was a member of family I.4 of ‘true lipase’ group. |
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