Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats
The study was conducted to evaluate the anti-peptic ulcer activity of ethanol extract of Camellia sinensis (green tea) induced by Helicobacter pylori in Sprague Dawley rats. The standard regimen of anti-peptic ulcer agents, clarithromycin 30 mg/kg, amoxicillin 30 mg/kg and esomeprazole 3 mg/kg use...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16009/ http://journalarticle.ukm.my/16009/1/18.pdf |
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| author | Marhanis Salihah Omar, Nur Nadiana Adnan, Endang Kumolosasi, Norazrina Azmi, Noor Safwah Damanhuri, Fhataheya Buang, |
| author_facet | Marhanis Salihah Omar, Nur Nadiana Adnan, Endang Kumolosasi, Norazrina Azmi, Noor Safwah Damanhuri, Fhataheya Buang, |
| author_sort | Marhanis Salihah Omar, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The study was conducted to evaluate the anti-peptic ulcer activity of ethanol extract of Camellia sinensis (green tea)
induced by Helicobacter pylori in Sprague Dawley rats. The standard regimen of anti-peptic ulcer agents, clarithromycin
30 mg/kg, amoxicillin 30 mg/kg and esomeprazole 3 mg/kg used as the positive control. The rats divided into 6 groups
(n = 6), normal control, vehicle control (2% of tragacanth suspension), positive control for preventive and treatment
groups, preventive and treatment groups were given 50 mg/kg orally of green tea extract for 14 days before and after
inoculation (as prevention and treatment, respectively) with 5×108 CFU/mL H. pylori suspension orally twice daily for
7 days. Anti-peptic ulcer activity was evaluated based on the pH of gastric juice, ulcer score and body weight of rats.
The results demonstrated that the use of green tea for prevention or treatment showed a significant difference (p<0.001)
in reducing the pH of gastric juice 3.88 ± 0.09 and 5.74 ± 0.08, respectively, compared to vehicle control 5.85 ± 0.12.
Meanwhile, vehicle control rats had high significantly reduced body weight when compared with all groups treated.
The preventive and the treatment groups showed significantly reduce in ulcer score to 0.50 ± 0.18 (p<0.01) and 0.83
± 0.10 (p<0.05), respectively, compared to vehicle control 1.50 ± 0.22. Meanwhile, it was comparable to the positive
control. In conclusion, H. pylori suspension 5×108 CFU/mL given orally twice daily for seven days able to induce ulcer
in Sprague Dawley rats, and the green tea extract 50 mg/kg was comparable to the standard regimen as anti-peptic
ulcer agent. |
| first_indexed | 2025-11-15T00:26:11Z |
| format | Article |
| id | oai:generic.eprints.org:16009 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:26:11Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:160092020-12-17T05:10:25Z http://journalarticle.ukm.my/16009/ Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats Marhanis Salihah Omar, Nur Nadiana Adnan, Endang Kumolosasi, Norazrina Azmi, Noor Safwah Damanhuri, Fhataheya Buang, The study was conducted to evaluate the anti-peptic ulcer activity of ethanol extract of Camellia sinensis (green tea) induced by Helicobacter pylori in Sprague Dawley rats. The standard regimen of anti-peptic ulcer agents, clarithromycin 30 mg/kg, amoxicillin 30 mg/kg and esomeprazole 3 mg/kg used as the positive control. The rats divided into 6 groups (n = 6), normal control, vehicle control (2% of tragacanth suspension), positive control for preventive and treatment groups, preventive and treatment groups were given 50 mg/kg orally of green tea extract for 14 days before and after inoculation (as prevention and treatment, respectively) with 5×108 CFU/mL H. pylori suspension orally twice daily for 7 days. Anti-peptic ulcer activity was evaluated based on the pH of gastric juice, ulcer score and body weight of rats. The results demonstrated that the use of green tea for prevention or treatment showed a significant difference (p<0.001) in reducing the pH of gastric juice 3.88 ± 0.09 and 5.74 ± 0.08, respectively, compared to vehicle control 5.85 ± 0.12. Meanwhile, vehicle control rats had high significantly reduced body weight when compared with all groups treated. The preventive and the treatment groups showed significantly reduce in ulcer score to 0.50 ± 0.18 (p<0.01) and 0.83 ± 0.10 (p<0.05), respectively, compared to vehicle control 1.50 ± 0.22. Meanwhile, it was comparable to the positive control. In conclusion, H. pylori suspension 5×108 CFU/mL given orally twice daily for seven days able to induce ulcer in Sprague Dawley rats, and the green tea extract 50 mg/kg was comparable to the standard regimen as anti-peptic ulcer agent. Penerbit Universiti Kebangsaan Malaysia 2020-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16009/1/18.pdf Marhanis Salihah Omar, and Nur Nadiana Adnan, and Endang Kumolosasi, and Norazrina Azmi, and Noor Safwah Damanhuri, and Fhataheya Buang, (2020) Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats. Sains Malaysiana, 49 (11). pp. 2793-2800. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid49bil11_2020/KandunganJilid49Bil11_2020.html |
| spellingShingle | Marhanis Salihah Omar, Nur Nadiana Adnan, Endang Kumolosasi, Norazrina Azmi, Noor Safwah Damanhuri, Fhataheya Buang, Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats |
| title | Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats |
| title_full | Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats |
| title_fullStr | Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats |
| title_full_unstemmed | Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats |
| title_short | Green tea (Camellia sinensis) extract reduces peptic ulcer induced by Helicobacter pylori in sprague dawley rats |
| title_sort | green tea (camellia sinensis) extract reduces peptic ulcer induced by helicobacter pylori in sprague dawley rats |
| url | http://journalarticle.ukm.my/16009/ http://journalarticle.ukm.my/16009/ http://journalarticle.ukm.my/16009/1/18.pdf |