High-dose edible bird’s nest extract (EBN) upregulates LDL-R via suppression of HMGCR gene expression in HepG2 cell lines
Edible bird’s nest (EBN) is an ancient food that had been consumed by Chinese people for well-being purposes. Nevertheless, the effect of EBN on cholesterol metabolism is poorly studied. Since the cholesterol is primarily being metabolized in the liver, HepG2 cell lines was selected as the model f...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15939/ http://journalarticle.ukm.my/15939/1/9.pdf |
| Summary: | Edible bird’s nest (EBN) is an ancient food that had been consumed by Chinese people for well-being purposes.
Nevertheless, the effect of EBN on cholesterol metabolism is poorly studied. Since the cholesterol is primarily being
metabolized in the liver, HepG2 cell lines was selected as the model for in-vitro preliminary assessment. Our EBN
extract (EBNE) showed 71% cell viability in HepG2 cell lines, even at the highest dose (1.5 mg/mL). Simultaneously, EBNE
was significantly up-regulated the LDL-R gene expression via suppression of HMGCR at the highest concentration.
Besides that, ACAT2 was up-regulated significantly to allow exogenous cholesterol storage. Expression analysis of
these genes were correlated with high immunofluorescence distribution of the LDL-R and LDs compared to other
treatment groups. Consistent with gene expression and immunofluorescence staining, intrahepatocellular cholesterol
concentration was increased significantly at the highest dose of EBNE. The EBNE had significantly improved
cholesterol metabolism in the HepG2 cell line via suppression of the HMGCR gene and subsequently up-regulated
LDL-R gene expression at the highest dose. Therefore, this study provides an insight to understand the effect of EBNE
in improving cholesterol metabolism. |
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