Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium
Acacia mangium is one of the most important species in forest plantation industry in Southeast Asia. Lignin is a complex polymer found in plant which has to be removed using harsh and toxic chemicals for the production of high quality paper. Understanding the complex pathway that underlies the reg...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15710/ http://journalarticle.ukm.my/15710/1/49_01_17.pdf |
| _version_ | 1848813865710649344 |
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| author | Azmah Abdul Latif, Sheh, May Tam Wickneswari Ratnam, |
| author_facet | Azmah Abdul Latif, Sheh, May Tam Wickneswari Ratnam, |
| author_sort | Azmah Abdul Latif, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Acacia mangium is one of the most important species in forest plantation industry in Southeast Asia. Lignin is a complex
polymer found in plant which has to be removed using harsh and toxic chemicals for the production of high quality paper.
Understanding the complex pathway that underlies the regulation of lignin biosynthetic genes requires in depth knowledge of
the genes involved and it’s regulatory elements. Using Thermal Asymmetric Interlaced PCR, a 770bp promoter of
pseudoCOMTAm and 619bp of AmCCR1 promoter were isolated. Bioinformatics analysis revealed the presence of cis acting
elements commonly found in lignin biosynthetic genes such as TATA box, CAAT box, W box, AC-I and AC-11 elements
on both promoters. |
| first_indexed | 2025-11-15T00:24:59Z |
| format | Article |
| id | oai:generic.eprints.org:15710 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:24:59Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:157102020-11-18T06:07:05Z http://journalarticle.ukm.my/15710/ Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium Azmah Abdul Latif, Sheh, May Tam Wickneswari Ratnam, Acacia mangium is one of the most important species in forest plantation industry in Southeast Asia. Lignin is a complex polymer found in plant which has to be removed using harsh and toxic chemicals for the production of high quality paper. Understanding the complex pathway that underlies the regulation of lignin biosynthetic genes requires in depth knowledge of the genes involved and it’s regulatory elements. Using Thermal Asymmetric Interlaced PCR, a 770bp promoter of pseudoCOMTAm and 619bp of AmCCR1 promoter were isolated. Bioinformatics analysis revealed the presence of cis acting elements commonly found in lignin biosynthetic genes such as TATA box, CAAT box, W box, AC-I and AC-11 elements on both promoters. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15710/1/49_01_17.pdf Azmah Abdul Latif, and Sheh, May Tam and Wickneswari Ratnam, (2020) Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium. Malaysian Applied Biology, 49 (1). pp. 153-158. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56 |
| spellingShingle | Azmah Abdul Latif, Sheh, May Tam Wickneswari Ratnam, Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium |
| title | Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium |
| title_full | Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium |
| title_fullStr | Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium |
| title_full_unstemmed | Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium |
| title_short | Characterisation of AmCCR1 and pseudoCOMTAm promoters in Acacia mangium |
| title_sort | characterisation of amccr1 and pseudocomtam promoters in acacia mangium |
| url | http://journalarticle.ukm.my/15710/ http://journalarticle.ukm.my/15710/ http://journalarticle.ukm.my/15710/1/49_01_17.pdf |