Immobilization of CLEA-lipase of Hevea brasiliensis onto magnetic nanoparticles for enhanced biocatalytic performance
Skim latex from Hevea brasiliensis consists of many useful proteins and enzymes that can be utilized to produce value-added products for industrial purposes. In this project, to enhance the properties of the recovered lipase from skim latex serum, this enzyme was immobilized via cross-linked enzym...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15709/ http://journalarticle.ukm.my/15709/1/49_01_16.pdf |
| Summary: | Skim latex from Hevea brasiliensis consists of many useful proteins and enzymes that can be utilized to produce value-added
products for industrial purposes. In this project, to enhance the properties of the recovered lipase from skim latex serum, this
enzyme was immobilized via cross-linked enzyme aggregates (CLEA) technology, and supported by magnetic nanoparticles
(MNP-CLEA-lipase). MNP-CLEA-lipase was prepared by chemical cross-linking of enzyme aggregates with amino
functionalized MNP, which can easily be separated from the spent media after use by magnetic field. The performances of
the newly produced MNP-CLEA-lipase were compared with its unsupported counterpart, CLEA-lipase. The optimum
conditions for the preparation of CLEA-lipase was carried out by using 45% saturated (NH4)2SO4 and 50 mM glutaraldehyde
(GA), whereas for MNP-CLEA-lipase, it was carried out by using 70% saturated (NH4)2SO4 and 60 mM GA. The optimum
pH changes from 6 to 8 when CLEA-lipase is supported, while achieving a maximum of 69.92% residual activity (RA)
compared to 20.66% for the unsupported CLEA-lipase. However, the optimum temperature for both are the same (35°C),
with MNP-CLEA-lipase achieving 85.89% RA compared to 31.91% for the unsupported ones. Stability studies carried out
on both showed that MNP-CLEA-lipase has higher thermal (25–60°C) and pH (5–10) stabilities compared to its unsupported
counterparts. As for the reusability of enzymes, MNP-CLEA-lipase retained 56.46% of residual activity after six cycles of
reuse. The FESEM results showed that the MNP-CLEA-lipase is a Type 2, which is less structured, and this has bearings
towards its activities. FTIR analysis showed the presence of Amide I and Amide II bands in MNP-CLEA-lipase, originating
from the newly formed cross-linked between the silanized MNP and CLEA-lipase. Overall, MNP supported CLEA-lipase
showed better performance compared to the unsupported biocatalyst. |
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