Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves
Dillenia suffruticosa or ‘Simpur bini’ is known to have ethnomedicinal properties and had been used traditionally to heal wounds, relieve fever and rheumatism. There has been limited studies carried out on this species, therefore, this study aims to evaluate the phytochemical contents, antioxidant...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15707/ http://journalarticle.ukm.my/15707/1/49_01_14.pdf |
| _version_ | 1848813864961966080 |
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| author | Farazimah Yakop, Malai Haniti Sheikh Abdul Hamid, Norhayati Ahmad, Mohamed Abdul Majid, Manoharan Karuppiah Pillai, Hussein Taha, |
| author_facet | Farazimah Yakop, Malai Haniti Sheikh Abdul Hamid, Norhayati Ahmad, Mohamed Abdul Majid, Manoharan Karuppiah Pillai, Hussein Taha, |
| author_sort | Farazimah Yakop, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Dillenia suffruticosa or ‘Simpur bini’ is known to have ethnomedicinal properties and had been used traditionally to heal
wounds, relieve fever and rheumatism. There has been limited studies carried out on this species, therefore, this study aims
to evaluate the phytochemical contents, antioxidant and antibacterial activities of aqueous extract, methanol extract and its
fractions obtained from the leaves of D. suffruticosa. The dried leaves were extracted using methanol before successive
solvent partitioning was carried out on the extract using hexane, chloroform, ethyl acetate and diethyl ether. In addition to
this, aqueous decoction was conducted. The antioxidant activities were determined using Total Phenolic Content (TPC), Total
Flavonoid Content (TFC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging methods. Phytochemical
screening had shown that most of the extracts and fractions contained alkaloids, steroids, phenolics, flavonoids and saponins.
The diethyl ether and ethyl acetate fractions showed higher TPC and TFC values. The diethyl ether and ethyl acetate fractions
also showed higher antioxidant activities determined via DPPH assay. Antibacterial activities determined using disc diffusion
assay showed the methanol extract and its fractions had antibacterial activity against Staphylococcus aureus, with the diethyl
ether fraction having comparable activity with the standard antibiotic streptomycin. However, inhibition against Bacillus subtilis
was only observed in hexane, chloroform and diethyl ether fractions. No inhibition was observed against Escherichia coli
and Pseudomonas aeruginosa. This study identified the diethyl ethyl and ethyl acetate fractions of D. suffruticosa leaves as
potential sources of bioactive compounds. Further investigations on the bioassay-guided isolation of bioactive compounds of
this species may lead to the discovery of new pharmaceutical or effective antimicrobial agents. |
| first_indexed | 2025-11-15T00:24:58Z |
| format | Article |
| id | oai:generic.eprints.org:15707 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:24:58Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:157072020-11-18T06:02:19Z http://journalarticle.ukm.my/15707/ Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves Farazimah Yakop, Malai Haniti Sheikh Abdul Hamid, Norhayati Ahmad, Mohamed Abdul Majid, Manoharan Karuppiah Pillai, Hussein Taha, Dillenia suffruticosa or ‘Simpur bini’ is known to have ethnomedicinal properties and had been used traditionally to heal wounds, relieve fever and rheumatism. There has been limited studies carried out on this species, therefore, this study aims to evaluate the phytochemical contents, antioxidant and antibacterial activities of aqueous extract, methanol extract and its fractions obtained from the leaves of D. suffruticosa. The dried leaves were extracted using methanol before successive solvent partitioning was carried out on the extract using hexane, chloroform, ethyl acetate and diethyl ether. In addition to this, aqueous decoction was conducted. The antioxidant activities were determined using Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging methods. Phytochemical screening had shown that most of the extracts and fractions contained alkaloids, steroids, phenolics, flavonoids and saponins. The diethyl ether and ethyl acetate fractions showed higher TPC and TFC values. The diethyl ether and ethyl acetate fractions also showed higher antioxidant activities determined via DPPH assay. Antibacterial activities determined using disc diffusion assay showed the methanol extract and its fractions had antibacterial activity against Staphylococcus aureus, with the diethyl ether fraction having comparable activity with the standard antibiotic streptomycin. However, inhibition against Bacillus subtilis was only observed in hexane, chloroform and diethyl ether fractions. No inhibition was observed against Escherichia coli and Pseudomonas aeruginosa. This study identified the diethyl ethyl and ethyl acetate fractions of D. suffruticosa leaves as potential sources of bioactive compounds. Further investigations on the bioassay-guided isolation of bioactive compounds of this species may lead to the discovery of new pharmaceutical or effective antimicrobial agents. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15707/1/49_01_14.pdf Farazimah Yakop, and Malai Haniti Sheikh Abdul Hamid, and Norhayati Ahmad, and Mohamed Abdul Majid, and Manoharan Karuppiah Pillai, and Hussein Taha, (2020) Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves. Malaysian Applied Biology, 49 (1). pp. 121-130. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56 |
| spellingShingle | Farazimah Yakop, Malai Haniti Sheikh Abdul Hamid, Norhayati Ahmad, Mohamed Abdul Majid, Manoharan Karuppiah Pillai, Hussein Taha, Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves |
| title | Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves |
| title_full | Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves |
| title_fullStr | Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves |
| title_full_unstemmed | Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves |
| title_short | Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves |
| title_sort | phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of dillenia suffruticosa leaves |
| url | http://journalarticle.ukm.my/15707/ http://journalarticle.ukm.my/15707/ http://journalarticle.ukm.my/15707/1/49_01_14.pdf |