Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization
Strenotrophomonas maltophilia B6 was a newly identified keratinolytic bacteria isolated from a landfill in North Sumatra. Keratinolytic activitiy of this strain was investigated through optimization and characterization of its enzymatic properties. Amino acid profiles were quantified using High Pe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15702/ http://journalarticle.ukm.my/15702/1/49_01_09.pdf |
| _version_ | 1848813863668023296 |
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| author | Mamangkey, Jendri Suryanto, Dwi Munir, Erman Mustopa, Apon Zaenal |
| author_facet | Mamangkey, Jendri Suryanto, Dwi Munir, Erman Mustopa, Apon Zaenal |
| author_sort | Mamangkey, Jendri |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Strenotrophomonas maltophilia B6 was a newly identified keratinolytic bacteria isolated from a landfill in North Sumatra.
Keratinolytic activitiy of this strain was investigated through optimization and characterization of its enzymatic properties.
Amino acid profiles were quantified using High Performance Liquid Chromatography (HPLC) after a 96-h of incubation
resulted in 2.2569 mg mL-1 amino acids from a 40-mL fermentation supplemented with 10 g/L chicken feathers. The
keratinolytic activities were enhanced through addition of 1% glucose yielded 67.41 ± 1.1 UmL-1 and 0.5% peptone yielded
90.24 ± 0.7 UmL-1. Keratinolytic activity enhanced with the addition of Ca+ (116%), Na+ (111%), Mg+ (110%) at 10 mM
concentration. Zymogram analysis showed a protein band in the 26 kDa of molecular mass, similar to serine protease. Physical
and chemical characteristics of degradation products were analyzed using Scanning Electron Microscope (SEM) and Fourier
Transform Infra Red (FTIR). Qualitatively, the optimum activity occurred at 96-h of incubation through SEM image analysis.
The FTIR peaks detected were peak 3747 cm-1 (-OH); peak 2972 cm-1 (asymmetric -CH3); peak 1641 cm-1 (amide I); peak
1438 cm-1 (amide II); peak 1092 cm-1 (-C-C-); peak 523 cm-1 (-S-S-). Thus, Strenotrophomonas maltophilia B6 may be
used as potential biodegradative bacterium to chicken feather wastes in nature. |
| first_indexed | 2025-11-15T00:24:57Z |
| format | Article |
| id | oai:generic.eprints.org:15702 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:24:57Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:157022020-11-18T05:52:37Z http://journalarticle.ukm.my/15702/ Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization Mamangkey, Jendri Suryanto, Dwi Munir, Erman Mustopa, Apon Zaenal Strenotrophomonas maltophilia B6 was a newly identified keratinolytic bacteria isolated from a landfill in North Sumatra. Keratinolytic activitiy of this strain was investigated through optimization and characterization of its enzymatic properties. Amino acid profiles were quantified using High Performance Liquid Chromatography (HPLC) after a 96-h of incubation resulted in 2.2569 mg mL-1 amino acids from a 40-mL fermentation supplemented with 10 g/L chicken feathers. The keratinolytic activities were enhanced through addition of 1% glucose yielded 67.41 ± 1.1 UmL-1 and 0.5% peptone yielded 90.24 ± 0.7 UmL-1. Keratinolytic activity enhanced with the addition of Ca+ (116%), Na+ (111%), Mg+ (110%) at 10 mM concentration. Zymogram analysis showed a protein band in the 26 kDa of molecular mass, similar to serine protease. Physical and chemical characteristics of degradation products were analyzed using Scanning Electron Microscope (SEM) and Fourier Transform Infra Red (FTIR). Qualitatively, the optimum activity occurred at 96-h of incubation through SEM image analysis. The FTIR peaks detected were peak 3747 cm-1 (-OH); peak 2972 cm-1 (asymmetric -CH3); peak 1641 cm-1 (amide I); peak 1438 cm-1 (amide II); peak 1092 cm-1 (-C-C-); peak 523 cm-1 (-S-S-). Thus, Strenotrophomonas maltophilia B6 may be used as potential biodegradative bacterium to chicken feather wastes in nature. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15702/1/49_01_09.pdf Mamangkey, Jendri and Suryanto, Dwi and Munir, Erman and Mustopa, Apon Zaenal (2020) Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization. Malaysian Applied Biology, 49 (1). pp. 75-86. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56 |
| spellingShingle | Mamangkey, Jendri Suryanto, Dwi Munir, Erman Mustopa, Apon Zaenal Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization |
| title | Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization |
| title_full | Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization |
| title_fullStr | Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization |
| title_full_unstemmed | Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization |
| title_short | Promoting keratinase activity from newly identified strain Strenotrophomonas maltophilia B6 through optimization and characterization |
| title_sort | promoting keratinase activity from newly identified strain strenotrophomonas maltophilia b6 through optimization and characterization |
| url | http://journalarticle.ukm.my/15702/ http://journalarticle.ukm.my/15702/ http://journalarticle.ukm.my/15702/1/49_01_09.pdf |