Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers
Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities in Indonesia. The purpose of this study was to analyze the genetic diversity of A. paniculata in five mainlands from Indonesia using ISSR and RAPD markers. A total of 50 ac...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15700/ http://journalarticle.ukm.my/15700/1/49_01_07.pdf |
| _version_ | 1848813863220281344 |
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| author | Arif, Muhammad Fauzi Subositi, Dyah Sari, Aniska Novita Aristya, Ganies Riza Lesmana, Indra Kasiamdari, Rina Sri |
| author_facet | Arif, Muhammad Fauzi Subositi, Dyah Sari, Aniska Novita Aristya, Ganies Riza Lesmana, Indra Kasiamdari, Rina Sri |
| author_sort | Arif, Muhammad Fauzi |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities
in Indonesia. The purpose of this study was to analyze the genetic diversity of A. paniculata in five mainlands from Indonesia
using ISSR and RAPD markers. A total of 50 accessions of A. paniculata were used, DNA was amplified using six ISSR
and six RAPD primers, generating 61 loci and 43 loci respectively. Genotype data scoring resulted in the percentage of
polymorphism, Polymorphic Information Content, Shannon’s Information Index and unbiased expected heterozygosity. The
genetic variation between the locations was measured with Principal Coordinate Analysis (PCoA) and pairwise Nei’s unbiased
genetic distance. The data from this study revealed that the variation and diversity from accessions within the same island
were low. The genetic variation was higher between accessions from different islands. Analysis of molecular variance
(AMOVA) was used to calculate the distribution of the variation between and within the A. paniculata population. The
results showed the variation derived from the same location was 34% and from a different location was 66%. Genetic diversity
provided information to help the further development and cultivation of A. paniculata in Indonesia. |
| first_indexed | 2025-11-15T00:24:57Z |
| format | Article |
| id | oai:generic.eprints.org:15700 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:24:57Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:157002020-11-16T23:37:33Z http://journalarticle.ukm.my/15700/ Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers Arif, Muhammad Fauzi Subositi, Dyah Sari, Aniska Novita Aristya, Ganies Riza Lesmana, Indra Kasiamdari, Rina Sri Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities in Indonesia. The purpose of this study was to analyze the genetic diversity of A. paniculata in five mainlands from Indonesia using ISSR and RAPD markers. A total of 50 accessions of A. paniculata were used, DNA was amplified using six ISSR and six RAPD primers, generating 61 loci and 43 loci respectively. Genotype data scoring resulted in the percentage of polymorphism, Polymorphic Information Content, Shannon’s Information Index and unbiased expected heterozygosity. The genetic variation between the locations was measured with Principal Coordinate Analysis (PCoA) and pairwise Nei’s unbiased genetic distance. The data from this study revealed that the variation and diversity from accessions within the same island were low. The genetic variation was higher between accessions from different islands. Analysis of molecular variance (AMOVA) was used to calculate the distribution of the variation between and within the A. paniculata population. The results showed the variation derived from the same location was 34% and from a different location was 66%. Genetic diversity provided information to help the further development and cultivation of A. paniculata in Indonesia. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15700/1/49_01_07.pdf Arif, Muhammad Fauzi and Subositi, Dyah and Sari, Aniska Novita and Aristya, Ganies Riza and Lesmana, Indra and Kasiamdari, Rina Sri (2020) Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers. Malaysian Applied Biology, 49 (1). pp. 61-68. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56 |
| spellingShingle | Arif, Muhammad Fauzi Subositi, Dyah Sari, Aniska Novita Aristya, Ganies Riza Lesmana, Indra Kasiamdari, Rina Sri Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers |
| title | Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers |
| title_full | Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers |
| title_fullStr | Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers |
| title_full_unstemmed | Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers |
| title_short | Genetic diversity of green chireta (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) from Indonesia based on ISSR and RAPD markers |
| title_sort | genetic diversity of green chireta (andrographis paniculata (burm.f.) wall. ex nees.) from indonesia based on issr and rapd markers |
| url | http://journalarticle.ukm.my/15700/ http://journalarticle.ukm.my/15700/ http://journalarticle.ukm.my/15700/1/49_01_07.pdf |