High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves

Lawsonia inermis (Henna), (Lythraceae), contains a high amount of phenolic compounds which could activate antioxidants to help reduce free radicals. In this study, the compound content found in the ethanol:water (80:20) extract of local Lawsonia inermis was determined using HPLC-QTOFMS. The 1 H-N...

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Main Authors: Muhammad Remy Othman, Rozana Othman, Abdul Aziz Ismail, Hazrina Hazni, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusoff, Khalijah Awang
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/15623/
http://journalarticle.ukm.my/15623/1/12.pdf
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author Muhammad Remy Othman,
Rozana Othman,
Abdul Aziz Ismail,
Hazrina Hazni,
Khadher Ahmad,
Munirah Abd Razzak,
Zulkifli Mohd Yusoff,
Khalijah Awang,
author_facet Muhammad Remy Othman,
Rozana Othman,
Abdul Aziz Ismail,
Hazrina Hazni,
Khadher Ahmad,
Munirah Abd Razzak,
Zulkifli Mohd Yusoff,
Khalijah Awang,
author_sort Muhammad Remy Othman,
building UKM Institutional Repository
collection Online Access
description Lawsonia inermis (Henna), (Lythraceae), contains a high amount of phenolic compounds which could activate antioxidants to help reduce free radicals. In this study, the compound content found in the ethanol:water (80:20) extract of local Lawsonia inermis was determined using HPLC-QTOFMS. The 1 H-NMR results were used to determine the peak that exists for the group compounds. Chromatographic peaks were detected and integrated by the MassHunter Acquisition B.07.00 for the Agilent TOF and QTOF and MassHunter Qualitative Analysis B.07.00. The ethanol:water (80:20) extract of L. inermis have shown, predominantly, the presence of phenolic compounds (coumarins, flavonoids, naphthalene, and gallic acid) which are highly glycosylated. The presence of compounds such as apiin, lawsone, apigenin, luteolin, cosmosiin, and p-coumaric acid were also found.
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institution Universiti Kebangasaan Malaysia
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publishDate 2020
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spelling oai:generic.eprints.org:156232020-11-11T16:32:49Z http://journalarticle.ukm.my/15623/ High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves Muhammad Remy Othman, Rozana Othman, Abdul Aziz Ismail, Hazrina Hazni, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusoff, Khalijah Awang, Lawsonia inermis (Henna), (Lythraceae), contains a high amount of phenolic compounds which could activate antioxidants to help reduce free radicals. In this study, the compound content found in the ethanol:water (80:20) extract of local Lawsonia inermis was determined using HPLC-QTOFMS. The 1 H-NMR results were used to determine the peak that exists for the group compounds. Chromatographic peaks were detected and integrated by the MassHunter Acquisition B.07.00 for the Agilent TOF and QTOF and MassHunter Qualitative Analysis B.07.00. The ethanol:water (80:20) extract of L. inermis have shown, predominantly, the presence of phenolic compounds (coumarins, flavonoids, naphthalene, and gallic acid) which are highly glycosylated. The presence of compounds such as apiin, lawsone, apigenin, luteolin, cosmosiin, and p-coumaric acid were also found. Penerbit Universiti Kebangsaan Malaysia 2020-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15623/1/12.pdf Muhammad Remy Othman, and Rozana Othman, and Abdul Aziz Ismail, and Hazrina Hazni, and Khadher Ahmad, and Munirah Abd Razzak, and Zulkifli Mohd Yusoff, and Khalijah Awang, (2020) High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves. Sains Malaysiana, 49 (7). pp. 1597-1613. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil7_2020/KandunganJilid49Bil7_2020.html
spellingShingle Muhammad Remy Othman,
Rozana Othman,
Abdul Aziz Ismail,
Hazrina Hazni,
Khadher Ahmad,
Munirah Abd Razzak,
Zulkifli Mohd Yusoff,
Khalijah Awang,
High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
title High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
title_full High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
title_fullStr High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
title_full_unstemmed High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
title_short High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
title_sort high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (hplc-qtofms) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
url http://journalarticle.ukm.my/15623/
http://journalarticle.ukm.my/15623/
http://journalarticle.ukm.my/15623/1/12.pdf