Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor
Persicaria minor has been recognised as a plant with high content of volatile organic compounds (VOC) especially terpenoid and green leaf volatile (GLV). Previous finding had showed signaling molecules such as abscisic acid (ABA) and methyl jasmonate (MeJA) can increase the VOC content in plant. I...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
|
| Online Access: | http://journalarticle.ukm.my/15460/ http://journalarticle.ukm.my/15460/1/4.pdf |
| _version_ | 1848813805662896128 |
|---|---|
| author | Abdul Fatah A. Samad, Nazaruddin, Nazaruddin Jaeyres Jani, Jaeyres Ismanizan Ismail, |
| author_facet | Abdul Fatah A. Samad, Nazaruddin, Nazaruddin Jaeyres Jani, Jaeyres Ismanizan Ismail, |
| author_sort | Abdul Fatah A. Samad, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Persicaria minor has been recognised as a plant with high content of volatile organic compounds (VOC) especially
terpenoid and green leaf volatile (GLV). Previous finding had showed signaling molecules such as abscisic acid
(ABA) and methyl jasmonate (MeJA) can increase the VOC content in plant. In this study, we performed next generation
sequencing (NGS) of small RNA to uncover miRNAs roles and their response to both phytohormones (ABA and MeJA)
in P. minor. For both ABA and MeJA treated P. minor, small RNA libraries containing 17,253,566 and 40,437,576 reads
were generated, respectively. In addition, 18,634,904 reads were generated in plant treated with sterile distilled
water which served as control. In these libraries, a total of 88 miRNAs were identified, comprising 41 known and
47 novel miRNAs. It was observed that 21 and 38 miRNAs were significantly regulated in ABA and MeJA libraries,
respectively. Four selected miRNAs related to VOC pathways were subjected to RT-qPCR analysis and found to
display diverse expression patterns with their targets. This study provides the initial framework for further exploration
of miRNA roles in ABA and MeJA responses. |
| first_indexed | 2025-11-15T00:24:02Z |
| format | Article |
| id | oai:generic.eprints.org:15460 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:24:02Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:154602020-10-30T04:17:30Z http://journalarticle.ukm.my/15460/ Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor Abdul Fatah A. Samad, Nazaruddin, Nazaruddin Jaeyres Jani, Jaeyres Ismanizan Ismail, Persicaria minor has been recognised as a plant with high content of volatile organic compounds (VOC) especially terpenoid and green leaf volatile (GLV). Previous finding had showed signaling molecules such as abscisic acid (ABA) and methyl jasmonate (MeJA) can increase the VOC content in plant. In this study, we performed next generation sequencing (NGS) of small RNA to uncover miRNAs roles and their response to both phytohormones (ABA and MeJA) in P. minor. For both ABA and MeJA treated P. minor, small RNA libraries containing 17,253,566 and 40,437,576 reads were generated, respectively. In addition, 18,634,904 reads were generated in plant treated with sterile distilled water which served as control. In these libraries, a total of 88 miRNAs were identified, comprising 41 known and 47 novel miRNAs. It was observed that 21 and 38 miRNAs were significantly regulated in ABA and MeJA libraries, respectively. Four selected miRNAs related to VOC pathways were subjected to RT-qPCR analysis and found to display diverse expression patterns with their targets. This study provides the initial framework for further exploration of miRNA roles in ABA and MeJA responses. Penerbit Universiti Kebangsaan Malaysia 2020-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15460/1/4.pdf Abdul Fatah A. Samad, and Nazaruddin, Nazaruddin and Jaeyres Jani, Jaeyres and Ismanizan Ismail, (2020) Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor. Sains Malaysiana, 49 (6). pp. 1245-1272. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil6_2020/KandunganJilid49Bil6_2020.html |
| spellingShingle | Abdul Fatah A. Samad, Nazaruddin, Nazaruddin Jaeyres Jani, Jaeyres Ismanizan Ismail, Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor |
| title | Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor |
| title_full | Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor |
| title_fullStr | Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor |
| title_full_unstemmed | Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor |
| title_short | Identification and analysis of microRNAs responsive to abscisic acid and methyl jasmonate treatments in Persicaria minor |
| title_sort | identification and analysis of micrornas responsive to abscisic acid and methyl jasmonate treatments in persicaria minor |
| url | http://journalarticle.ukm.my/15460/ http://journalarticle.ukm.my/15460/ http://journalarticle.ukm.my/15460/1/4.pdf |