Alterations of cholesterol lowering-related proteins in the serum of hypercholesterolemic-induced rats treated with Ficus deltoidea
Cardiovascular disease (CVD) is one of the major non-communicable diseases causing cardiac dysfunction and heart failure. In Malaysia, hypercholesterolemia or high blood cholesterol is known as CVD’s main risk factor. Currently, there is a paradigm shift of interest in Eastern medicines as alterna...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15396/ http://journalarticle.ukm.my/15396/1/10.pdf |
| Summary: | Cardiovascular disease (CVD) is one of the major non-communicable diseases causing cardiac dysfunction and heart
failure. In Malaysia, hypercholesterolemia or high blood cholesterol is known as CVD’s main risk factor. Currently, there
is a paradigm shift of interest in Eastern medicines as alternative prevention approaches and therapeutic cholesterol
control treatment. Ficus deltoidea (Mas Cotek) is amongst the eastern medicinal plants traditionally consumed either as
prevention or treatment for various diseases such as hypertension, diabetes and hypercholesterolemia. This study utilised
the proteomic application in determining the changes in a series of proteins that are being differentially expressed in
response to F. deltoidea treatment of hypercholesterolemic-induced rats. Identification and quantification of proteins were
conducted by using quantitative techniques, wherein two dimensional gel electrophoresis (2D-GE) along with Progenesis
Samespots image analysis software and Matrix-assisted laser desorption/ionization Time-of-flight/Time-of-flight (MALDI
ToF/ToF) mass spectrometry were utilised. Our results demonstrated that 14 protein spots had displayed alteration
in their abundance at a minimum of 1.5 fold. Majority of these identified proteins were involved in lipid metabolism
and immune system processes. These variables include apolipoprotein A1 (Apo A1), apolipoprotein E (Apo E), retinol
binding protein 4 (RBP4), haptoglobin (HP), complement factor C3 (C3), complement factor C1s (C1s), transthyretin
(TTR), ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3), prominin 1(PROM1) and plasminogen (PLG).
Serum proteome analysis has unravelled the understanding on the mechanism of cholesterol reducing by F. deltoidea
via augmentation of cholesterol biosynthesis that enhanced bile acids excretion, causing lowered cholesterol absorption
in hypercholesterolemic rats. |
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