Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction
Chlorella sp. microalgae contain phenolic compounds with good functional properties. In this study, two extraction methods, soxhlet and subcritical water extraction (SWE) were applied and compared in terms of phenolic compounds recovery from Chlorella sp. microalgae and characterization of the phe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15347/ http://journalarticle.ukm.my/15347/1/5.pdf |
| _version_ | 1848813778301353984 |
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| author | Siti Maisurah Zakaria, Siti Mazlina Mustapa Kamal, Razif Harun, Rozita Omar, Shamsul Izhar Siajam, |
| author_facet | Siti Maisurah Zakaria, Siti Mazlina Mustapa Kamal, Razif Harun, Rozita Omar, Shamsul Izhar Siajam, |
| author_sort | Siti Maisurah Zakaria, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Chlorella sp. microalgae contain phenolic compounds with good functional properties. In this study, two extraction
methods, soxhlet and subcritical water extraction (SWE) were applied and compared in terms of phenolic compounds
recovery from Chlorella sp. microalgae and characterization of the phenolic acid components. Phenolic acid analysis
demonstrated that the main components of the Chlorella sp. extracts were ferulic, caffeic and p-coumaric acids. The
comparative study indicated that SWE gave higher extraction yield compared to conventional soxhlet method. High
recoveries of phenolic acids were obtained at 175 °C with 3.20, 3.05 and 3.33 mg/100 g of ferulic, p-coumaric and
caffeic acid, respectively, compared to soxhlet extraction using methanol with 2.10, 2.29 and 2.37 mg/100 g of ferulic,
p-coumaric and caffeic acid, respectively. This proved that subcritical water treatment could effectively be used for
the release of phenolic acids from Chlorella sp. using safe and green solvent. Analysis by Fourier transform infrared
spectroscopy (FTIR) was also performed to observe the effect of subcritical water on the functional groups of the extracts.
The current study demonstrated that SWE provided a better way of utilising Chlorella sp. as a source of phenolic acids
and natural antioxidants. |
| first_indexed | 2025-11-15T00:23:36Z |
| format | Article |
| id | oai:generic.eprints.org:15347 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:23:36Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:153472020-10-16T00:24:09Z http://journalarticle.ukm.my/15347/ Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction Siti Maisurah Zakaria, Siti Mazlina Mustapa Kamal, Razif Harun, Rozita Omar, Shamsul Izhar Siajam, Chlorella sp. microalgae contain phenolic compounds with good functional properties. In this study, two extraction methods, soxhlet and subcritical water extraction (SWE) were applied and compared in terms of phenolic compounds recovery from Chlorella sp. microalgae and characterization of the phenolic acid components. Phenolic acid analysis demonstrated that the main components of the Chlorella sp. extracts were ferulic, caffeic and p-coumaric acids. The comparative study indicated that SWE gave higher extraction yield compared to conventional soxhlet method. High recoveries of phenolic acids were obtained at 175 °C with 3.20, 3.05 and 3.33 mg/100 g of ferulic, p-coumaric and caffeic acid, respectively, compared to soxhlet extraction using methanol with 2.10, 2.29 and 2.37 mg/100 g of ferulic, p-coumaric and caffeic acid, respectively. This proved that subcritical water treatment could effectively be used for the release of phenolic acids from Chlorella sp. using safe and green solvent. Analysis by Fourier transform infrared spectroscopy (FTIR) was also performed to observe the effect of subcritical water on the functional groups of the extracts. The current study demonstrated that SWE provided a better way of utilising Chlorella sp. as a source of phenolic acids and natural antioxidants. Penerbit Universiti Kebangsaan Malaysia 2020-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15347/1/5.pdf Siti Maisurah Zakaria, and Siti Mazlina Mustapa Kamal, and Razif Harun, and Rozita Omar, and Shamsul Izhar Siajam, (2020) Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction. Sains Malaysiana, 49 (4). pp. 765-774. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil4_2020/KandunganJilid49Bil4_2020.html |
| spellingShingle | Siti Maisurah Zakaria, Siti Mazlina Mustapa Kamal, Razif Harun, Rozita Omar, Shamsul Izhar Siajam, Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_full | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_fullStr | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_full_unstemmed | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_short | Characterization on phenolic acids and antioxidant activity of Chlorella sp. microalgae using subcritical water extraction |
| title_sort | characterization on phenolic acids and antioxidant activity of chlorella sp. microalgae using subcritical water extraction |
| url | http://journalarticle.ukm.my/15347/ http://journalarticle.ukm.my/15347/ http://journalarticle.ukm.my/15347/1/5.pdf |