Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells
Ascorbic acid has two isoforms, i.e., L-ascorbic acid which exists naturally and D-ascorbic acid which only can be found in synthetic form. This study aimed to determine the cytotoxic effect of L- and D- ascorbic acid on primary cell, i.e. murine and human suspension cells of peripheral blood. Murin...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/15190/ http://journalarticle.ukm.my/15190/1/ARTIKEL%2014.pdf |
| _version_ | 1848813736670789632 |
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| author | Intan Zarina Zainol Abidin, Thanaletchumi Manogaran, Shahrul Hisham Zainal Ariffin, |
| author_facet | Intan Zarina Zainol Abidin, Thanaletchumi Manogaran, Shahrul Hisham Zainal Ariffin, |
| author_sort | Intan Zarina Zainol Abidin, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Ascorbic acid has two isoforms, i.e., L-ascorbic acid which exists naturally and D-ascorbic acid which only can be found in synthetic form. This study aimed to determine the cytotoxic effect of L- and D- ascorbic acid on primary cell, i.e. murine and human suspension cells of peripheral blood. Murine and human suspension blood cells were obtained through density gradient centrifugation using Ficoll-Paque™ PLUS. Non-adherent cells were identified after 7 days in culture and seeded at 1 × 105 cells/mL. Both newly isolated primary cells were analyzed for in vitro proliferation ability for 7 days. The cells were treated with L- and D-ascorbic acid at concentrations 30, 50, and 90 μg/mL followed by Trypan blue viability assay at day 0, 3, 7, and 14. Cells cultured in the complete medium were represented as control. Murine and human suspension blood cells showed round morphology and significant increase of viable cells after 7 days in complete medium. Both cells treated with L-ascorbic acid exhibited low cytotoxic effect at 30, 50, and 90 μg/mL concentrations. In addition, murine blood cells treated with D-ascorbic acid caused total population cell death in all three concentrations at day 14, whereas human suspension blood cells only exhibited total population cell death at higher concentration, i.e. 90 μg/mL. In conclusion, L-ascorbic acid exhibited minimal cytotoxic effect on both primary cell sources as compared to lethal effect of D-ascorbic acid treatment. |
| first_indexed | 2025-11-15T00:22:56Z |
| format | Article |
| id | oai:generic.eprints.org:15190 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:22:56Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:151902020-09-14T02:08:52Z http://journalarticle.ukm.my/15190/ Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells Intan Zarina Zainol Abidin, Thanaletchumi Manogaran, Shahrul Hisham Zainal Ariffin, Ascorbic acid has two isoforms, i.e., L-ascorbic acid which exists naturally and D-ascorbic acid which only can be found in synthetic form. This study aimed to determine the cytotoxic effect of L- and D- ascorbic acid on primary cell, i.e. murine and human suspension cells of peripheral blood. Murine and human suspension blood cells were obtained through density gradient centrifugation using Ficoll-Paque™ PLUS. Non-adherent cells were identified after 7 days in culture and seeded at 1 × 105 cells/mL. Both newly isolated primary cells were analyzed for in vitro proliferation ability for 7 days. The cells were treated with L- and D-ascorbic acid at concentrations 30, 50, and 90 μg/mL followed by Trypan blue viability assay at day 0, 3, 7, and 14. Cells cultured in the complete medium were represented as control. Murine and human suspension blood cells showed round morphology and significant increase of viable cells after 7 days in complete medium. Both cells treated with L-ascorbic acid exhibited low cytotoxic effect at 30, 50, and 90 μg/mL concentrations. In addition, murine blood cells treated with D-ascorbic acid caused total population cell death in all three concentrations at day 14, whereas human suspension blood cells only exhibited total population cell death at higher concentration, i.e. 90 μg/mL. In conclusion, L-ascorbic acid exhibited minimal cytotoxic effect on both primary cell sources as compared to lethal effect of D-ascorbic acid treatment. Penerbit Universiti Kebangsaan Malaysia 2020-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15190/1/ARTIKEL%2014.pdf Intan Zarina Zainol Abidin, and Thanaletchumi Manogaran, and Shahrul Hisham Zainal Ariffin, (2020) Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells. Sains Malaysiana, 49 (3). pp. 593-602. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil3_2020/KandunganJilid49Bil3_2020.html |
| spellingShingle | Intan Zarina Zainol Abidin, Thanaletchumi Manogaran, Shahrul Hisham Zainal Ariffin, Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells |
| title | Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells |
| title_full | Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells |
| title_fullStr | Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells |
| title_full_unstemmed | Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells |
| title_short | Cytotoxicity of L- and D-ascorbic acid on murine and human suspension peripheral blood cells |
| title_sort | cytotoxicity of l- and d-ascorbic acid on murine and human suspension peripheral blood cells |
| url | http://journalarticle.ukm.my/15190/ http://journalarticle.ukm.my/15190/ http://journalarticle.ukm.my/15190/1/ARTIKEL%2014.pdf |