Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels
Histological observation has always been important in the study of sentinels. In this research, histological differences in the liver and kidney of two wild populations of Fejervarya limnocharis exposed to different environmental cadmium levels were observed. Liver and kidney samples from 30 rice...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/15077/ http://journalarticle.ukm.my/15077/1/38409-121643-1-PB.pdf |
| _version_ | 1848813710580121600 |
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| author | Mohd Sham Othman, Robson, Mark Gregory Wichase Khonsue, Jirarach Kitana, Kumthorn Thirakhupt, Noppadon Kitana, |
| author_facet | Mohd Sham Othman, Robson, Mark Gregory Wichase Khonsue, Jirarach Kitana, Kumthorn Thirakhupt, Noppadon Kitana, |
| author_sort | Mohd Sham Othman, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Histological observation has always been important in the study of sentinels. In this research, histological differences
in the liver and kidney of two wild populations of Fejervarya limnocharis exposed to different environmental cadmium
levels were observed. Liver and kidney samples from 30 rice frogs caught from reference site (Mae Pa) and contaminated
site (Mae Tao) were sectioned and stained with Haemotoxylin and Eosin and then observed under light microscope.
It was found that liver sections from frogs caught from Mae Tao had higher hepatic macro-melanophage count (MMC)
(0.949 ± 0.267 cells/1000 μm2) as compared to those from Mae Pa (0.672 ± 0.299 cells/1000 μm2). Other observable
histological features found in frog’s liver from Mae Tao include possible necrotic areas, cellular swellings and chromatin
margination. The kidney sections from frogs caught from Mae Tao showed the presence of tumor-like cell aggregation
and hemorrhage. However, the proportion of tumor-like cell aggregation and hemorrhage were not significantly different
than were expected from random occurrences. The result of the histological study revealed that F. limnocharis caught
from Mae Tao had higher hepatic MMC and higher prevalence of renal tumor-like aggregation and renal hemorrhage
than the rice frogs caught from Mae Pa. Cellular swelling, possible necrotic area and possible apoptotic cell could also
be observed in the liver of contaminated frogs. |
| first_indexed | 2025-11-15T00:22:31Z |
| format | Article |
| id | oai:generic.eprints.org:15077 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:22:31Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:150772020-08-24T07:37:02Z http://journalarticle.ukm.my/15077/ Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels Mohd Sham Othman, Robson, Mark Gregory Wichase Khonsue, Jirarach Kitana, Kumthorn Thirakhupt, Noppadon Kitana, Histological observation has always been important in the study of sentinels. In this research, histological differences in the liver and kidney of two wild populations of Fejervarya limnocharis exposed to different environmental cadmium levels were observed. Liver and kidney samples from 30 rice frogs caught from reference site (Mae Pa) and contaminated site (Mae Tao) were sectioned and stained with Haemotoxylin and Eosin and then observed under light microscope. It was found that liver sections from frogs caught from Mae Tao had higher hepatic macro-melanophage count (MMC) (0.949 ± 0.267 cells/1000 μm2) as compared to those from Mae Pa (0.672 ± 0.299 cells/1000 μm2). Other observable histological features found in frog’s liver from Mae Tao include possible necrotic areas, cellular swellings and chromatin margination. The kidney sections from frogs caught from Mae Tao showed the presence of tumor-like cell aggregation and hemorrhage. However, the proportion of tumor-like cell aggregation and hemorrhage were not significantly different than were expected from random occurrences. The result of the histological study revealed that F. limnocharis caught from Mae Tao had higher hepatic MMC and higher prevalence of renal tumor-like aggregation and renal hemorrhage than the rice frogs caught from Mae Pa. Cellular swelling, possible necrotic area and possible apoptotic cell could also be observed in the liver of contaminated frogs. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15077/1/38409-121643-1-PB.pdf Mohd Sham Othman, and Robson, Mark Gregory and Wichase Khonsue, and Jirarach Kitana, and Kumthorn Thirakhupt, and Noppadon Kitana, (2019) Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels. Jurnal Sains Kesihatan Malaysia, 17 ((SI)). pp. 65-71. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/1247 |
| spellingShingle | Mohd Sham Othman, Robson, Mark Gregory Wichase Khonsue, Jirarach Kitana, Kumthorn Thirakhupt, Noppadon Kitana, Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels |
| title | Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels |
| title_full | Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels |
| title_fullStr | Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels |
| title_full_unstemmed | Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels |
| title_short | Histological differences in the livers and kidneys of two populations of rice frog (Fejervarya limnocharis) naturally exposed to different environmental cadmium levels |
| title_sort | histological differences in the livers and kidneys of two populations of rice frog (fejervarya limnocharis) naturally exposed to different environmental cadmium levels |
| url | http://journalarticle.ukm.my/15077/ http://journalarticle.ukm.my/15077/ http://journalarticle.ukm.my/15077/1/38409-121643-1-PB.pdf |