Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes
Functional mushroom polysaccharides have been known to the Asian traditional medicine for many years. The anti-obesity activity of mushroom polysaccharides has been illustrated both in vivo and in vitro. However, the effect of pure polysaccharides on isolated fat cells has not been demonstrated. In...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
|
| Online Access: | http://journalarticle.ukm.my/14736/ http://journalarticle.ukm.my/14736/1/ARTIKEL%2012.pdf |
| _version_ | 1848813630332600320 |
|---|---|
| author | Decha Sermwittayawong, Kulwanit Patninan, Chanawee Jakkawanpitak, Somruthai Phothiphiphit, Siwarutt Boonyarattanakalin, Masashi Inafuku, Hirosuke Oku, Kusumarn Noipha, Nongporn Hutadilok-Towatana, |
| author_facet | Decha Sermwittayawong, Kulwanit Patninan, Chanawee Jakkawanpitak, Somruthai Phothiphiphit, Siwarutt Boonyarattanakalin, Masashi Inafuku, Hirosuke Oku, Kusumarn Noipha, Nongporn Hutadilok-Towatana, |
| author_sort | Decha Sermwittayawong, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Functional mushroom polysaccharides have been known to the Asian traditional medicine for many years. The anti-obesity activity of mushroom polysaccharides has been illustrated both in vivo and in vitro. However, the effect of pure polysaccharides on isolated fat cells has not been demonstrated. In this study, we used hot water and ammonium oxalate to extract polysaccharides from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] and subsequently purified with enzymatic digestion, solvent extraction and ion-exchange chromatography. One of the fractions, 7S1-1, has the highest yield and purity and contains polysaccharides with the molecular weight around 500 kDa. In addition, Fourier transformed infrared spectroscopy (FTIR) and monosaccharide composition analyses showed that this 7S1-1 sample contains mostly β-glucan and some mannan. We find that the 7S1-1 fraction does not affect the total amount of triglycerides in the 3T3-L1 adipocytes but promotes a release of glycerol of the fat cells, suggesting that the fraction does not inhibit adipogenesis but stimulates lipolysis. Laminarin, a small β-glucan, confers similar results to the 7S1-1 sample, but it could not stimulate lipolysis as well as the 7S1-1 sample. Therefore, these results suggest a potential anti-obesity activity of the mushroom polysaccharides. |
| first_indexed | 2025-11-15T00:21:15Z |
| format | Article |
| id | oai:generic.eprints.org:14736 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:21:15Z |
| publishDate | 2020 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:147362020-06-17T07:54:03Z http://journalarticle.ukm.my/14736/ Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes Decha Sermwittayawong, Kulwanit Patninan, Chanawee Jakkawanpitak, Somruthai Phothiphiphit, Siwarutt Boonyarattanakalin, Masashi Inafuku, Hirosuke Oku, Kusumarn Noipha, Nongporn Hutadilok-Towatana, Functional mushroom polysaccharides have been known to the Asian traditional medicine for many years. The anti-obesity activity of mushroom polysaccharides has been illustrated both in vivo and in vitro. However, the effect of pure polysaccharides on isolated fat cells has not been demonstrated. In this study, we used hot water and ammonium oxalate to extract polysaccharides from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] and subsequently purified with enzymatic digestion, solvent extraction and ion-exchange chromatography. One of the fractions, 7S1-1, has the highest yield and purity and contains polysaccharides with the molecular weight around 500 kDa. In addition, Fourier transformed infrared spectroscopy (FTIR) and monosaccharide composition analyses showed that this 7S1-1 sample contains mostly β-glucan and some mannan. We find that the 7S1-1 fraction does not affect the total amount of triglycerides in the 3T3-L1 adipocytes but promotes a release of glycerol of the fat cells, suggesting that the fraction does not inhibit adipogenesis but stimulates lipolysis. Laminarin, a small β-glucan, confers similar results to the 7S1-1 sample, but it could not stimulate lipolysis as well as the 7S1-1 sample. Therefore, these results suggest a potential anti-obesity activity of the mushroom polysaccharides. Penerbit Universiti Kebangsaan Malaysia 2020-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14736/1/ARTIKEL%2012.pdf Decha Sermwittayawong, and Kulwanit Patninan, and Chanawee Jakkawanpitak, and Somruthai Phothiphiphit, and Siwarutt Boonyarattanakalin, and Masashi Inafuku, and Hirosuke Oku, and Kusumarn Noipha, and Nongporn Hutadilok-Towatana, (2020) Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes. Sains Malaysiana, 49 (1). pp. 103-112. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil1_2020/KandunganJilid49Bil1_2020.html |
| spellingShingle | Decha Sermwittayawong, Kulwanit Patninan, Chanawee Jakkawanpitak, Somruthai Phothiphiphit, Siwarutt Boonyarattanakalin, Masashi Inafuku, Hirosuke Oku, Kusumarn Noipha, Nongporn Hutadilok-Towatana, Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes |
| title | Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes |
| title_full | Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes |
| title_fullStr | Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes |
| title_full_unstemmed | Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes |
| title_short | Effect of purified soluble polysaccharides extracted from gray oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] on 3t3-L1 adipocytes |
| title_sort | effect of purified soluble polysaccharides extracted from gray oyster mushroom [pleurotus sajor-caju (fr.) sing.] on 3t3-l1 adipocytes |
| url | http://journalarticle.ukm.my/14736/ http://journalarticle.ukm.my/14736/ http://journalarticle.ukm.my/14736/1/ARTIKEL%2012.pdf |