Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum
The natural water resources contaminate high levels of nutrients, especially nitrogen and phosphorus, which can stimulate algae to grow rapidly. Microcystis spp., Cylindrospermopsis spp. and Anabaena spp. are dominant species in water. However, those algae can produce harmful toxins, called microc...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14714/ http://journalarticle.ukm.my/14714/1/48_04_10.pdf |
| _version_ | 1848813624106156032 |
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| author | Natthaphon Chaosuan, Sirikae Pongswat, Manida Chorum, |
| author_facet | Natthaphon Chaosuan, Sirikae Pongswat, Manida Chorum, |
| author_sort | Natthaphon Chaosuan, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The natural water resources contaminate high levels of nutrients, especially nitrogen and phosphorus, which can stimulate
algae to grow rapidly. Microcystis spp., Cylindrospermopsis spp. and Anabaena spp. are dominant species in water. However,
those algae can produce harmful toxins, called microcystin LR (MC-LR) affecting human and animal health. Therefore, this
research aimed to eliminate MC-LR by using lactic acid bacteria. Lactobacillus plantalum TISTR 854 (LP) was used in this
experiment. The effects of glucose concentrations (0, 1 and 3% w/v), pH (4.0, 7.0, and 10.0), and temperatures (25, 30 and
35°C) on LP removal activity were studied. LP was cultured in Lactobacillus MRS medium, centrifuged, and re-suspended
in phosphate buffered saline at different pH. Different glucose contents and MC-LR were then added into the cell solutions,
before incubate at the mentioned temperatures for 24 hr. The concentrations of MC-LR were then analyzed by Protein
Phosphatase Inhibition Assay. The results were expressed as %MC-LR removing. The result showed that LP could reduce
MC-LR. The optimal condition for MC-LR was found 1% w/v glucose, pH 7.0, and incubation temperature at 35°C, showed
77±0.6% of MC-LR removal. The study indicated that LP can be used to remove microcystin from water. |
| first_indexed | 2025-11-15T00:21:09Z |
| format | Article |
| id | oai:generic.eprints.org:14714 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:21:09Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:147142020-06-03T00:38:15Z http://journalarticle.ukm.my/14714/ Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum Natthaphon Chaosuan, Sirikae Pongswat, Manida Chorum, The natural water resources contaminate high levels of nutrients, especially nitrogen and phosphorus, which can stimulate algae to grow rapidly. Microcystis spp., Cylindrospermopsis spp. and Anabaena spp. are dominant species in water. However, those algae can produce harmful toxins, called microcystin LR (MC-LR) affecting human and animal health. Therefore, this research aimed to eliminate MC-LR by using lactic acid bacteria. Lactobacillus plantalum TISTR 854 (LP) was used in this experiment. The effects of glucose concentrations (0, 1 and 3% w/v), pH (4.0, 7.0, and 10.0), and temperatures (25, 30 and 35°C) on LP removal activity were studied. LP was cultured in Lactobacillus MRS medium, centrifuged, and re-suspended in phosphate buffered saline at different pH. Different glucose contents and MC-LR were then added into the cell solutions, before incubate at the mentioned temperatures for 24 hr. The concentrations of MC-LR were then analyzed by Protein Phosphatase Inhibition Assay. The results were expressed as %MC-LR removing. The result showed that LP could reduce MC-LR. The optimal condition for MC-LR was found 1% w/v glucose, pH 7.0, and incubation temperature at 35°C, showed 77±0.6% of MC-LR removal. The study indicated that LP can be used to remove microcystin from water. Penerbit Universiti Kebangsaan Malaysia 2019-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14714/1/48_04_10.pdf Natthaphon Chaosuan, and Sirikae Pongswat, and Manida Chorum, (2019) Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum. Malaysian Applied Biology, 48 (4). pp. 75-82. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=946&catid=59:current-view&Itemid=56 |
| spellingShingle | Natthaphon Chaosuan, Sirikae Pongswat, Manida Chorum, Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum |
| title | Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum |
| title_full | Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum |
| title_fullStr | Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum |
| title_full_unstemmed | Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum |
| title_short | Removal of cyanotoxin microcystin-LR by Lactobacillus plantarum |
| title_sort | removal of cyanotoxin microcystin-lr by lactobacillus plantarum |
| url | http://journalarticle.ukm.my/14714/ http://journalarticle.ukm.my/14714/ http://journalarticle.ukm.my/14714/1/48_04_10.pdf |