Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran
Leptin is a polypeptide which is mostly secreted by the white adipose tissue and a little by gastrointestinal tract and placenta, plays an important role for controlling body weight, feed intake, immunity, milk production, and reproduction. The aim of this study was to investigate the exon 2 of th...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14691/ http://journalarticle.ukm.my/14691/1/48_03_10.pdf |
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| author | Vazir, Narges Esmaeili Aliabad Koshkooieh, Ali Esmaeilizadeh Mehrjerdi, Ahmad Aytolahi Mohammadabadi, Mohammadreza Babenko, Olena Ivanivna Bushtruk, Maryna Vitaliivna Tkachenko, Serhii Vasyliovych Stavetska, Ruslana Volodymyrivna Klopenko, Nataliia Ihorivna |
| author_facet | Vazir, Narges Esmaeili Aliabad Koshkooieh, Ali Esmaeilizadeh Mehrjerdi, Ahmad Aytolahi Mohammadabadi, Mohammadreza Babenko, Olena Ivanivna Bushtruk, Maryna Vitaliivna Tkachenko, Serhii Vasyliovych Stavetska, Ruslana Volodymyrivna Klopenko, Nataliia Ihorivna |
| author_sort | Vazir, Narges Esmaeili Aliabad |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Leptin is a polypeptide which is mostly secreted by the white adipose tissue and a little by gastrointestinal tract and placenta,
plays an important role for controlling body weight, feed intake, immunity, milk production, and reproduction. The aim of
this study was to investigate the exon 2 of the leptin gene polymorphism in Iranian wild and domestic goats using PCRRFLP. Blood samples were collected from 14 wild and domestic goat breeds including (Cashmere Abadeh, Torki-Ghahghaei,
Naeini, Robati, Nadoushani, Adani, Shahrbabaki, Birjandi, China goat, Sannen, Pakistani, Raeini cashmere, Najdi, and Wild
goat) and then genomic DNA was extracted. A 152 bp fragment from exon 2 of the leptin gene was amplified. PCR products
were digested with Hinf I restriction enzymes and was separated and visualized on the agarose gels. From possible three
genotypes (TT, TC, and CC), only two genotypes TT and CC were observed in 14 studied domestic and wild populations
with the genotype frequency of 95% and 5% respectively. The number of observed alleles, number of effective alleles, Nei’s
Index and Shanon’s Index were 2, 1.10, 0.10 and 0.20 respectively. The studied populations were not found to be in HardyWeinberg equilibrium. Our investigation demonstrated that TT genotype and T allele had a very high frequency (0.95) in
studied goats. Hence, it can be concluded that this finding can provide the basis for selection when considering evolution and
differentiation among breeds, however, further studies should be carried out on a larger population of different domestic and
wild breeds to verify the final conclusions. |
| first_indexed | 2025-11-15T00:21:03Z |
| format | Article |
| id | oai:generic.eprints.org:14691 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:21:03Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:146912020-05-27T01:19:57Z http://journalarticle.ukm.my/14691/ Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran Vazir, Narges Esmaeili Aliabad Koshkooieh, Ali Esmaeilizadeh Mehrjerdi, Ahmad Aytolahi Mohammadabadi, Mohammadreza Babenko, Olena Ivanivna Bushtruk, Maryna Vitaliivna Tkachenko, Serhii Vasyliovych Stavetska, Ruslana Volodymyrivna Klopenko, Nataliia Ihorivna Leptin is a polypeptide which is mostly secreted by the white adipose tissue and a little by gastrointestinal tract and placenta, plays an important role for controlling body weight, feed intake, immunity, milk production, and reproduction. The aim of this study was to investigate the exon 2 of the leptin gene polymorphism in Iranian wild and domestic goats using PCRRFLP. Blood samples were collected from 14 wild and domestic goat breeds including (Cashmere Abadeh, Torki-Ghahghaei, Naeini, Robati, Nadoushani, Adani, Shahrbabaki, Birjandi, China goat, Sannen, Pakistani, Raeini cashmere, Najdi, and Wild goat) and then genomic DNA was extracted. A 152 bp fragment from exon 2 of the leptin gene was amplified. PCR products were digested with Hinf I restriction enzymes and was separated and visualized on the agarose gels. From possible three genotypes (TT, TC, and CC), only two genotypes TT and CC were observed in 14 studied domestic and wild populations with the genotype frequency of 95% and 5% respectively. The number of observed alleles, number of effective alleles, Nei’s Index and Shanon’s Index were 2, 1.10, 0.10 and 0.20 respectively. The studied populations were not found to be in HardyWeinberg equilibrium. Our investigation demonstrated that TT genotype and T allele had a very high frequency (0.95) in studied goats. Hence, it can be concluded that this finding can provide the basis for selection when considering evolution and differentiation among breeds, however, further studies should be carried out on a larger population of different domestic and wild breeds to verify the final conclusions. Penerbit Universiti Kebangsaan Malaysia 2019-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14691/1/48_03_10.pdf Vazir, Narges Esmaeili Aliabad and Koshkooieh, Ali Esmaeilizadeh and Mehrjerdi, Ahmad Aytolahi and Mohammadabadi, Mohammadreza and Babenko, Olena Ivanivna and Bushtruk, Maryna Vitaliivna and Tkachenko, Serhii Vasyliovych and Stavetska, Ruslana Volodymyrivna and Klopenko, Nataliia Ihorivna (2019) Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran. Malaysian Applied Biology, 48 (3). pp. 85-93. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=924&catid=59:current-view&Itemid=56 |
| spellingShingle | Vazir, Narges Esmaeili Aliabad Koshkooieh, Ali Esmaeilizadeh Mehrjerdi, Ahmad Aytolahi Mohammadabadi, Mohammadreza Babenko, Olena Ivanivna Bushtruk, Maryna Vitaliivna Tkachenko, Serhii Vasyliovych Stavetska, Ruslana Volodymyrivna Klopenko, Nataliia Ihorivna Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran |
| title | Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran |
| title_full | Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran |
| title_fullStr | Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran |
| title_full_unstemmed | Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran |
| title_short | Comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in Iran |
| title_sort | comparison of genetic diversity of leptin gene between wild goat and domestic goat breeds in iran |
| url | http://journalarticle.ukm.my/14691/ http://journalarticle.ukm.my/14691/ http://journalarticle.ukm.my/14691/1/48_03_10.pdf |