Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology
Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting special features such as non-toxic, outstandi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14662/ http://journalarticle.ukm.my/14662/1/48_02_04.pdf |
| _version_ | 1848813610025877504 |
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| author | Farihin Azhari, Uswatun Hasanah Zaidan, Syahida Ahmad, Siti Salwa Abd. Gani, |
| author_facet | Farihin Azhari, Uswatun Hasanah Zaidan, Syahida Ahmad, Siti Salwa Abd. Gani, |
| author_sort | Farihin Azhari, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to
the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting
special features such as non-toxic, outstanding moisturizing action and most importantly the absence of oily texture. In this
study, Nigella sativa oil-based ferulate ester was synthesized via transesterification of Nigella sativa oil (NSO) and ethyl
ferulate (EF) in the presence of immobilized lipase, Lipozyme RM IM from Rhizomucor miehei. A five-level-four-factor
central composite rotatable design (CCRD) from response surface methodology (RSM) was applied to study the influence of
synthesis parameters: lipase dosage (50-80 mg), temperature (40-60°C), substrate ratio of NSO:EF (2.5:1-5.5:1 w/w) and
time (3-7 hr) aiming for the maximum percentage yield of ester. The optimized synthesis conditions were lipase dosage of 67
mg, temperature of 56°C, substrate ratio (NSO:EF) of 4.4:1 w/w and time of 4 hr. The maximum production of ester obtained
was 49.87% which is acceptable with the predicted value of 48.82%. |
| first_indexed | 2025-11-15T00:20:55Z |
| format | Article |
| id | oai:generic.eprints.org:14662 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:20:55Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:146622020-05-21T13:37:47Z http://journalarticle.ukm.my/14662/ Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology Farihin Azhari, Uswatun Hasanah Zaidan, Syahida Ahmad, Siti Salwa Abd. Gani, Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting special features such as non-toxic, outstanding moisturizing action and most importantly the absence of oily texture. In this study, Nigella sativa oil-based ferulate ester was synthesized via transesterification of Nigella sativa oil (NSO) and ethyl ferulate (EF) in the presence of immobilized lipase, Lipozyme RM IM from Rhizomucor miehei. A five-level-four-factor central composite rotatable design (CCRD) from response surface methodology (RSM) was applied to study the influence of synthesis parameters: lipase dosage (50-80 mg), temperature (40-60°C), substrate ratio of NSO:EF (2.5:1-5.5:1 w/w) and time (3-7 hr) aiming for the maximum percentage yield of ester. The optimized synthesis conditions were lipase dosage of 67 mg, temperature of 56°C, substrate ratio (NSO:EF) of 4.4:1 w/w and time of 4 hr. The maximum production of ester obtained was 49.87% which is acceptable with the predicted value of 48.82%. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14662/1/48_02_04.pdf Farihin Azhari, and Uswatun Hasanah Zaidan, and Syahida Ahmad, and Siti Salwa Abd. Gani, (2019) Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology. Malaysian Applied Biology, 48 (2). pp. 27-31. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=921&catid=59:current-view&Itemid=56 |
| spellingShingle | Farihin Azhari, Uswatun Hasanah Zaidan, Syahida Ahmad, Siti Salwa Abd. Gani, Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_full | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_fullStr | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_full_unstemmed | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_short | Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| title_sort | optimization of nigella sativa oil-based ferulate ester synthesis using response surface methodology |
| url | http://journalarticle.ukm.my/14662/ http://journalarticle.ukm.my/14662/ http://journalarticle.ukm.my/14662/1/48_02_04.pdf |