Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)

Coptotermes curvignathus is a subterranean termite species that vigorously feed on living-tree. The cellulose, hemicelluloses, lignin components in the wood are too robust for many organisms to break down and extract energy from but they serve as main carbon and energy sources for C. curvignathus. T...

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Main Authors: Hoe, Pik Kheng, King, Jie Hung, Patricia, Ong, Kian Huat, Bong, Choon Fah, Joseph, Nor Muhammad Mahadi
Format: Article
Language:English
Published: Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/14656/
http://journalarticle.ukm.my/14656/1/33916-116258-1-PB.pdf
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author Hoe, Pik Kheng
King, Jie Hung
Patricia,
Ong, Kian Huat
Bong, Choon Fah
Joseph,
Nor Muhammad Mahadi,
author_facet Hoe, Pik Kheng
King, Jie Hung
Patricia,
Ong, Kian Huat
Bong, Choon Fah
Joseph,
Nor Muhammad Mahadi,
author_sort Hoe, Pik Kheng
building UKM Institutional Repository
collection Online Access
description Coptotermes curvignathus is a subterranean termite species that vigorously feed on living-tree. The cellulose, hemicelluloses, lignin components in the wood are too robust for many organisms to break down and extract energy from but they serve as main carbon and energy sources for C. curvignathus. The ability of this subterranean termite thrive on wood diet may attribute by its array of laccase repertoire. Laccases are known for many functions including detoxification of plant tissues via xenobiotic pathway, and most importantly for termite is lignin modification. This paper highlighted the type of laccases produced by C. curvignathus based on transcriptomic data that were generated from 500 termites’ digestive system using Illumina HiSeq 2000. Raw data was trimmed and assembled by SOLEXAQA and Bowtie before loaded into Gene Ontology based data mining software, Blast2GO (B2G). The result showed that, C. curvignathus expressed diverse laccase genes that were phylogenetically similar to other termites’ laccases and distinctly related to fungal or bacterial laccases. Other than providing laccase genes sequences for further gene and enzyme characterization, the result of this study is the first insight into C. curvignathus laccase repertoire that is important to elucidate how C. curvignathus could digest wood efficiently from either intact or partially hydrolyzed wood.
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spelling oai:generic.eprints.org:146562020-05-19T04:27:18Z http://journalarticle.ukm.my/14656/ Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae) Hoe, Pik Kheng King, Jie Hung Patricia, Ong, Kian Huat Bong, Choon Fah Joseph, Nor Muhammad Mahadi, Coptotermes curvignathus is a subterranean termite species that vigorously feed on living-tree. The cellulose, hemicelluloses, lignin components in the wood are too robust for many organisms to break down and extract energy from but they serve as main carbon and energy sources for C. curvignathus. The ability of this subterranean termite thrive on wood diet may attribute by its array of laccase repertoire. Laccases are known for many functions including detoxification of plant tissues via xenobiotic pathway, and most importantly for termite is lignin modification. This paper highlighted the type of laccases produced by C. curvignathus based on transcriptomic data that were generated from 500 termites’ digestive system using Illumina HiSeq 2000. Raw data was trimmed and assembled by SOLEXAQA and Bowtie before loaded into Gene Ontology based data mining software, Blast2GO (B2G). The result showed that, C. curvignathus expressed diverse laccase genes that were phylogenetically similar to other termites’ laccases and distinctly related to fungal or bacterial laccases. Other than providing laccase genes sequences for further gene and enzyme characterization, the result of this study is the first insight into C. curvignathus laccase repertoire that is important to elucidate how C. curvignathus could digest wood efficiently from either intact or partially hydrolyzed wood. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2019-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14656/1/33916-116258-1-PB.pdf Hoe, Pik Kheng and King, Jie Hung and Patricia, and Ong, Kian Huat and Bong, Choon Fah and Joseph, and Nor Muhammad Mahadi, (2019) Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae). Serangga, 24 (2). pp. 169-197. ISSN 1394-5130 http://ejournals.ukm.my/serangga/issue/view/1237/showToc
spellingShingle Hoe, Pik Kheng
King, Jie Hung
Patricia,
Ong, Kian Huat
Bong, Choon Fah
Joseph,
Nor Muhammad Mahadi,
Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_full Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_fullStr Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_full_unstemmed Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_short Laccases repertoire of a subterranean termite Coptotermes curvignathus holmgren (Blattodea: Rhinotermitidae)
title_sort laccases repertoire of a subterranean termite coptotermes curvignathus holmgren (blattodea: rhinotermitidae)
url http://journalarticle.ukm.my/14656/
http://journalarticle.ukm.my/14656/
http://journalarticle.ukm.my/14656/1/33916-116258-1-PB.pdf