Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia
Rickettsia is a gram-negative, non-motile, and obligate intracellular bacterium that usually associated with arthropods vectors such as ticks, fleas, lice and mites. These bacteria have the ability to cause diseases in humans, however, in Malaysia, knowledge on the prevalence and distribution of the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14636/ http://journalarticle.ukm.my/14636/1/30967-104144-1-PB.pdf |
| _version_ | 1848813603086401536 |
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| author | Siti Nabilah Ishak, Lim, Fang Shiang Khoo, Jing Jing Farah Shafawati Mohd-Taib, |
| author_facet | Siti Nabilah Ishak, Lim, Fang Shiang Khoo, Jing Jing Farah Shafawati Mohd-Taib, |
| author_sort | Siti Nabilah Ishak, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Rickettsia is a gram-negative, non-motile, and obligate intracellular bacterium that usually associated with arthropods vectors such as ticks, fleas, lice and mites. These bacteria have the ability to cause diseases in humans, however, in Malaysia, knowledge on the prevalence and distribution of these bacteria mainly focused on humans and information of these bacteria in small mammal hosts is limited. Thus, this study aims to investigate the presence of Rickettsia sp. in the DNA of tick’s samples collected from small mammals in three different recreational forests in Selangor, Malaysia. Sampling was conducted in which 200 cage traps were set up randomly along streams and forest trails for five nights. A total of 106 fully engorged and adult ticks were collected from 23 individuals of seven small mammals host species. All samples were tested for Rickettsia bacteria based on polymerase chain reaction (PCR) using partial 17kDa antigen gene. The PCR results obtained from this study showed no infestation of Rickettsia sp. in all tick samples. Our findings revealed that none of the tick samples from these forests’ sites were infected with Rickettsia pathogen, however, more intense and extensive surveillance for Rickettsia sp. from other tick species is still necessary for greater geographical areas across Malaysia. |
| first_indexed | 2025-11-15T00:20:49Z |
| format | Article |
| id | oai:generic.eprints.org:14636 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:20:49Z |
| publishDate | 2019 |
| publisher | Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:146362020-05-17T01:43:24Z http://journalarticle.ukm.my/14636/ Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia Siti Nabilah Ishak, Lim, Fang Shiang Khoo, Jing Jing Farah Shafawati Mohd-Taib, Rickettsia is a gram-negative, non-motile, and obligate intracellular bacterium that usually associated with arthropods vectors such as ticks, fleas, lice and mites. These bacteria have the ability to cause diseases in humans, however, in Malaysia, knowledge on the prevalence and distribution of these bacteria mainly focused on humans and information of these bacteria in small mammal hosts is limited. Thus, this study aims to investigate the presence of Rickettsia sp. in the DNA of tick’s samples collected from small mammals in three different recreational forests in Selangor, Malaysia. Sampling was conducted in which 200 cage traps were set up randomly along streams and forest trails for five nights. A total of 106 fully engorged and adult ticks were collected from 23 individuals of seven small mammals host species. All samples were tested for Rickettsia bacteria based on polymerase chain reaction (PCR) using partial 17kDa antigen gene. The PCR results obtained from this study showed no infestation of Rickettsia sp. in all tick samples. Our findings revealed that none of the tick samples from these forests’ sites were infected with Rickettsia pathogen, however, more intense and extensive surveillance for Rickettsia sp. from other tick species is still necessary for greater geographical areas across Malaysia. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2019-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14636/1/30967-104144-1-PB.pdf Siti Nabilah Ishak, and Lim, Fang Shiang and Khoo, Jing Jing and Farah Shafawati Mohd-Taib, (2019) Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia. Serangga, 24 (1). pp. 116-125. ISSN 1394-5130 http://ejournals.ukm.my/serangga/issue/view/1198/showToc |
| spellingShingle | Siti Nabilah Ishak, Lim, Fang Shiang Khoo, Jing Jing Farah Shafawati Mohd-Taib, Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia |
| title | Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia |
| title_full | Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia |
| title_fullStr | Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia |
| title_full_unstemmed | Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia |
| title_short | Screening of Rickettsia sp. in ticks (Acari: Ixodidae) collected from small mammals in three recreational forests in Selangor, Malaysia |
| title_sort | screening of rickettsia sp. in ticks (acari: ixodidae) collected from small mammals in three recreational forests in selangor, malaysia |
| url | http://journalarticle.ukm.my/14636/ http://journalarticle.ukm.my/14636/ http://journalarticle.ukm.my/14636/1/30967-104144-1-PB.pdf |