Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB)
The final goal of this study is to make a modern systematic-based inventory of amylolytic bacterial isolates producing of bioplastic Poly-β-hydroxybutyrate (PHB) from sago starch substrate. The identity of three local bacterial isolates was examined in this study, using a polyphasic approach. A data...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14454/ http://journalarticle.ukm.my/14454/1/07%20Nur%20Arfa%20Yanti.pdf |
| _version_ | 1848813553600954368 |
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| author | Nur Arfa Yanti, Sembiring, L. Margino, S. H. Muhiddin, Nurhayani Ahmad, Sitti Wirdhana Ardiansyah, |
| author_facet | Nur Arfa Yanti, Sembiring, L. Margino, S. H. Muhiddin, Nurhayani Ahmad, Sitti Wirdhana Ardiansyah, |
| author_sort | Nur Arfa Yanti, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The final goal of this study is to make a modern systematic-based inventory of amylolytic bacterial isolates producing of bioplastic Poly-β-hydroxybutyrate (PHB) from sago starch substrate. The identity of three local bacterial isolates was examined in this study, using a polyphasic approach. A data set based on phenotypic characteristics, namely morphological, physiological, biochemical and chemical character, namely whole cells protein profiles using SDS-PAGE method, together with phylogenetic studies based on 16S rRNA sequences was used to identified by polyphasic approach. Phenotypic characteristics of 3 local bacterial isolates and 4 reference strains to members of genus Bacillus was analyzed by numerical analysis using MVSP 3,1 program to determine the value of similarity. Based on the preliminary characterization of the profile matching method showed that the three isolates of bacteria producing PHB namely PSA10, PPK5 and PPK6 are members of the genus Bacillus. The results of numerical analysis based on phenotypic characteristic and chemical character of the three bacterial isolates producing PHB with reference strains showed that the PSA10 isolate bacterial identical with Bacillus megaterium, PPK5 isolate identical with Bacillus subtilis and PPK6 isolate identical with Bacillus cereus, and these results also support by the molecular phylogenetic analysis. Therefore, the polyphasic taxonomy is an effective approach to uncover the identity of the novel bacterial isolates. |
| first_indexed | 2025-11-15T00:20:01Z |
| format | Article |
| id | oai:generic.eprints.org:14454 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:20:01Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:144542020-04-21T03:01:54Z http://journalarticle.ukm.my/14454/ Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB) Nur Arfa Yanti, Sembiring, L. Margino, S. H. Muhiddin, Nurhayani Ahmad, Sitti Wirdhana Ardiansyah, The final goal of this study is to make a modern systematic-based inventory of amylolytic bacterial isolates producing of bioplastic Poly-β-hydroxybutyrate (PHB) from sago starch substrate. The identity of three local bacterial isolates was examined in this study, using a polyphasic approach. A data set based on phenotypic characteristics, namely morphological, physiological, biochemical and chemical character, namely whole cells protein profiles using SDS-PAGE method, together with phylogenetic studies based on 16S rRNA sequences was used to identified by polyphasic approach. Phenotypic characteristics of 3 local bacterial isolates and 4 reference strains to members of genus Bacillus was analyzed by numerical analysis using MVSP 3,1 program to determine the value of similarity. Based on the preliminary characterization of the profile matching method showed that the three isolates of bacteria producing PHB namely PSA10, PPK5 and PPK6 are members of the genus Bacillus. The results of numerical analysis based on phenotypic characteristic and chemical character of the three bacterial isolates producing PHB with reference strains showed that the PSA10 isolate bacterial identical with Bacillus megaterium, PPK5 isolate identical with Bacillus subtilis and PPK6 isolate identical with Bacillus cereus, and these results also support by the molecular phylogenetic analysis. Therefore, the polyphasic taxonomy is an effective approach to uncover the identity of the novel bacterial isolates. Penerbit Universiti Kebangsaan Malaysia 2019-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14454/1/07%20Nur%20Arfa%20Yanti.pdf Nur Arfa Yanti, and Sembiring, L. and Margino, S. and H. Muhiddin, Nurhayani and Ahmad, Sitti Wirdhana and Ardiansyah, (2019) Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB). Sains Malaysiana, 48 (12). pp. 2663-2673. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil12_2019/KandunganJilid48Bil12_2019.html |
| spellingShingle | Nur Arfa Yanti, Sembiring, L. Margino, S. H. Muhiddin, Nurhayani Ahmad, Sitti Wirdhana Ardiansyah, Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB) |
| title | Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB) |
| title_full | Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB) |
| title_fullStr | Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB) |
| title_full_unstemmed | Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB) |
| title_short | Polyphasic identification of amylolytic bacteria producing bioplastic Poly-β-hydroxybutyrate (PHB) |
| title_sort | polyphasic identification of amylolytic bacteria producing bioplastic poly-β-hydroxybutyrate (phb) |
| url | http://journalarticle.ukm.my/14454/ http://journalarticle.ukm.my/14454/ http://journalarticle.ukm.my/14454/1/07%20Nur%20Arfa%20Yanti.pdf |