Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation
This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue rege...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/14347/ http://journalarticle.ukm.my/14347/1/14%20Asrar%20Elahi.pdf |
| _version_ | 1848813528485462016 |
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| author | Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid, |
| author_facet | Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid, |
| author_sort | Elahi, Asrar |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue regeneration such as low immunogenicity, anti-fibrosis, anti-inflammation, and a rich extracellular matrix component. Commercially available HPDLFs were seeded onto the HAM, and the attachment and proliferation of the cells were observed through scanning electron microscopy (SEM) and histological analysis. Cell viability was assessed using the alamarBlue® proliferation assay at days 1, 3, 7, 14 and 21. Histologically, the HPDLFs showed a monolayer to multilayer attachment onto the HAM from day 1 to day 7. The SEM analysis demonstrated that the HPDLFs had attached appropriately onto the HAM surface at day 1 to day 3, and began overlapping at day 7, while maintaining their flat shape. However, by days 14 and 21, there was an alteration in the morphology of the cells, where they later became rounded. The proliferation assay showed that the viability of the HPDLFs on the HAM had increased significantly from day 1 to day 7 (p=0.012), but later showed significant reduction at day 14 (p=0.002) and day 21 (p=0.005). In conclusion, this study showed that the HAM was able to function well as a scaffold for HPDLFs within 7 days, and thus, it can be a promising scaffold for periodontal regeneration. However, the behaviour of the cells in relation to the membrane over longer culture duration warrants further investigation. |
| first_indexed | 2025-11-15T00:19:37Z |
| format | Article |
| id | oai:generic.eprints.org:14347 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:19:37Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:143472020-03-03T08:01:52Z http://journalarticle.ukm.my/14347/ Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid, This study was aimed at evaluating the ability of the human amniotic membrane (HAM) to act as a scaffold for the growth of the main cells in periodontal regeneration, human periodontal ligament fibroblasts (HPDLFs). The HAM has many biological properties that are suitable for periodontal tissue regeneration such as low immunogenicity, anti-fibrosis, anti-inflammation, and a rich extracellular matrix component. Commercially available HPDLFs were seeded onto the HAM, and the attachment and proliferation of the cells were observed through scanning electron microscopy (SEM) and histological analysis. Cell viability was assessed using the alamarBlue® proliferation assay at days 1, 3, 7, 14 and 21. Histologically, the HPDLFs showed a monolayer to multilayer attachment onto the HAM from day 1 to day 7. The SEM analysis demonstrated that the HPDLFs had attached appropriately onto the HAM surface at day 1 to day 3, and began overlapping at day 7, while maintaining their flat shape. However, by days 14 and 21, there was an alteration in the morphology of the cells, where they later became rounded. The proliferation assay showed that the viability of the HPDLFs on the HAM had increased significantly from day 1 to day 7 (p=0.012), but later showed significant reduction at day 14 (p=0.002) and day 21 (p=0.005). In conclusion, this study showed that the HAM was able to function well as a scaffold for HPDLFs within 7 days, and thus, it can be a promising scaffold for periodontal regeneration. However, the behaviour of the cells in relation to the membrane over longer culture duration warrants further investigation. Penerbit Universiti Kebangsaan Malaysia 2019-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14347/1/14%20Asrar%20Elahi.pdf Elahi, Asrar and Haslina Taib, and Zurairah Berahim, and Azlina Ahmad, and Suzina Sheikh Ab Hamid, (2019) Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation. Sains Malaysiana, 48 (9). pp. 1927-1935. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil9_2019/KandunganJilid48Bil9_2019.html |
| spellingShingle | Elahi, Asrar Haslina Taib, Zurairah Berahim, Azlina Ahmad, Suzina Sheikh Ab Hamid, Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation |
| title | Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation |
| title_full | Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation |
| title_fullStr | Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation |
| title_full_unstemmed | Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation |
| title_short | Evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation |
| title_sort | evaluation of the human amniotic membrane as a scaffold for periodontal ligament fibroblast attachment and proliferation |
| url | http://journalarticle.ukm.my/14347/ http://journalarticle.ukm.my/14347/ http://journalarticle.ukm.my/14347/1/14%20Asrar%20Elahi.pdf |