Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)

This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp- specific primer. The morphology and histology of ovary of RPW were also studied and examined by...

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Main Authors: Wan Nurul ‘Ain W.M.N., Nurul Wahida O., Yaakop S., Norefrina Shafinaz M.N.
Format: Article
Language:English
Published: Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/13962/
http://journalarticle.ukm.my/13962/1/25775-86999-1-PB.pdf
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author Wan Nurul ‘Ain W.M.N.,
Nurul Wahida O.,
Yaakop S.,
Norefrina Shafinaz M.N.,
author_facet Wan Nurul ‘Ain W.M.N.,
Nurul Wahida O.,
Yaakop S.,
Norefrina Shafinaz M.N.,
author_sort Wan Nurul ‘Ain W.M.N.,
building UKM Institutional Repository
collection Online Access
description This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp- specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system.
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institution Universiti Kebangasaan Malaysia
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language English
last_indexed 2025-11-15T00:17:56Z
publishDate 2018
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recordtype eprints
repository_type Digital Repository
spelling oai:generic.eprints.org:139622020-01-17T09:01:01Z http://journalarticle.ukm.my/13962/ Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae) Wan Nurul ‘Ain W.M.N., Nurul Wahida O., Yaakop S., Norefrina Shafinaz M.N., This study was conducted to assess intracellular bacteria Wolbachia in the ovaries of female Red Palm Weevil (RPW), Rhynchophorus ferrugineus from three populations in Malaysia by means of PCR using wsp- specific primer. The morphology and histology of ovary of RPW were also studied and examined by stereo microscope LEICA EZ4 HD that was equipped with LAS EZ software and light microscope Zeiss Axio Scope with iSolutionLite software respectively. We found that the adult female had two pairs of ovaries, lateral oviduct, common oviduct, bursa copulatrix, spermatheca and vagina. Histological study of the ovariole revealed that it is categorised under polytrophic ovariole. The ovariole is divided into four regions, the terminal filament, the germarium, the vitellarium and the stalk or calyx. Besides, the infection status showed that all three populations were not infected with Wolbachia. Our result suggests that no infection of Wolbachia in RPW reproductive system. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13962/1/25775-86999-1-PB.pdf Wan Nurul ‘Ain W.M.N., and Nurul Wahida O., and Yaakop S., and Norefrina Shafinaz M.N., (2018) Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae). Serangga, 23 (2(SI)). pp. 183-193. ISSN 1394-5130 http://ejournal.ukm.my/serangga/issue/view/1133/showToc
spellingShingle Wan Nurul ‘Ain W.M.N.,
Nurul Wahida O.,
Yaakop S.,
Norefrina Shafinaz M.N.,
Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_full Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_fullStr Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_full_unstemmed Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_short Morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, Rhynchophorus ferrugineus (coleoptera: dryophthoridae)
title_sort morphology and histology of reproductive organ and first screening of wolbachia in the ovary of red palm weevil, rhynchophorus ferrugineus (coleoptera: dryophthoridae)
url http://journalarticle.ukm.my/13962/
http://journalarticle.ukm.my/13962/
http://journalarticle.ukm.my/13962/1/25775-86999-1-PB.pdf