The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma
Metastasis of tumour cell greatly contributes to the cause of mortality. Tumour-associated macrophage (TAM) and the intercellular adhesion molecule 1 (ICAM-1) were associated with metastases of breast carcinoma. However, the relationship between lymphatic vessel densities and invasion with TAM and I...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/13744/ http://journalarticle.ukm.my/13744/1/19%20Chong%20Choi%20Yen.pdf |
| _version_ | 1848813362898534400 |
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| author | Chong, Choi Yen Sabreena Safuan, |
| author_facet | Chong, Choi Yen Sabreena Safuan, |
| author_sort | Chong, Choi Yen |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Metastasis of tumour cell greatly contributes to the cause of mortality. Tumour-associated macrophage (TAM) and the intercellular adhesion molecule 1 (ICAM-1) were associated with metastases of breast carcinoma. However, the relationship between lymphatic vessel densities and invasion with TAM and ICAM-1 remained unclear. The aim of this study was to investigate the relationship of lymphovascular densities and invasions with patient’s clinicopathological data. The roles of TAM and ICAM-1 influencing lymphovascular invasions were also investigated. Haematoxylin and eosin (H&E) and immunohistochemical (IHC) staining on a consecutive section of 37 formalin fixed-paraffin embedded (FFPE) breast invasive carcinoma samples were carried out. The D2-40, CD34, CD163, and ICAM-1 antibodies were used to stain lymphatic vessel, blood vessel, TAM, and ICAM-1 receptor, respectively. The total lymphatic vessel density (LVD) was significantly reduced on increased tumor size (p=0.045). The increase of intra-tumoral LVD and lymphatic vessel invasion (LVI) was significantly associated with human epidermal growth factor receptor 2 (HER2) negative status (p=0.022 and p=0.05, respectively). The percentage of LVI was higher than blood vessel invasion (BVI) in 18.5%. Lymphovascular invasions detected in H&E were missed in 49.76% compared with those detected in IHC-stained tissues (206/410). ICAM-1 scores were significantly associated with non-triple negative breast cancer (non-TNBC) (p=0.008). ICAM-1 is significantly overexpressed on non-TNBC sample. Therefore, ICAM-1 might be clinically useful as a targeted molecule for non-TNBC patients. In histological reporting, in addition to H&E staining, IHC staining using D2-40 and CD34 should be considered to increase the accuracy of diagnosis. |
| first_indexed | 2025-11-15T00:16:59Z |
| format | Article |
| id | oai:generic.eprints.org:13744 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:16:59Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
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| spelling | oai:generic.eprints.org:137442019-11-29T10:30:03Z http://journalarticle.ukm.my/13744/ The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma Chong, Choi Yen Sabreena Safuan, Metastasis of tumour cell greatly contributes to the cause of mortality. Tumour-associated macrophage (TAM) and the intercellular adhesion molecule 1 (ICAM-1) were associated with metastases of breast carcinoma. However, the relationship between lymphatic vessel densities and invasion with TAM and ICAM-1 remained unclear. The aim of this study was to investigate the relationship of lymphovascular densities and invasions with patient’s clinicopathological data. The roles of TAM and ICAM-1 influencing lymphovascular invasions were also investigated. Haematoxylin and eosin (H&E) and immunohistochemical (IHC) staining on a consecutive section of 37 formalin fixed-paraffin embedded (FFPE) breast invasive carcinoma samples were carried out. The D2-40, CD34, CD163, and ICAM-1 antibodies were used to stain lymphatic vessel, blood vessel, TAM, and ICAM-1 receptor, respectively. The total lymphatic vessel density (LVD) was significantly reduced on increased tumor size (p=0.045). The increase of intra-tumoral LVD and lymphatic vessel invasion (LVI) was significantly associated with human epidermal growth factor receptor 2 (HER2) negative status (p=0.022 and p=0.05, respectively). The percentage of LVI was higher than blood vessel invasion (BVI) in 18.5%. Lymphovascular invasions detected in H&E were missed in 49.76% compared with those detected in IHC-stained tissues (206/410). ICAM-1 scores were significantly associated with non-triple negative breast cancer (non-TNBC) (p=0.008). ICAM-1 is significantly overexpressed on non-TNBC sample. Therefore, ICAM-1 might be clinically useful as a targeted molecule for non-TNBC patients. In histological reporting, in addition to H&E staining, IHC staining using D2-40 and CD34 should be considered to increase the accuracy of diagnosis. Penerbit Universiti Kebangsaan Malaysia 2019-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13744/1/19%20Chong%20Choi%20Yen.pdf Chong, Choi Yen and Sabreena Safuan, (2019) The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma. Sains Malaysiana, 48 (7). pp. 1503-1511. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil7_2019/KandunganJilid48Bil7_2019.html |
| spellingShingle | Chong, Choi Yen Sabreena Safuan, The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma |
| title | The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma |
| title_full | The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma |
| title_fullStr | The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma |
| title_full_unstemmed | The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma |
| title_short | The association of ICAM-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma |
| title_sort | association of icam-1 detected by immunohistochemical staining with triple negative and non-triple negative breast carcinoma |
| url | http://journalarticle.ukm.my/13744/ http://journalarticle.ukm.my/13744/ http://journalarticle.ukm.my/13744/1/19%20Chong%20Choi%20Yen.pdf |