In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa
Imipenem-resistant Pseudomonas aeruginosa (IRPA) infection is a serious problem in hospitals. Combination therapy is an alternative treatment for this infection. In this study, the in vitro activities of amikacin, aztreonam, ceftazidime, ciprofloxacin, colistin, imipenem, and piperacillin/tazobactam...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/13628/ http://journalarticle.ukm.my/13628/1/15%20Wanutsanun%20Tunyapanit.pdf |
| _version_ | 1848813330182963200 |
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| author | Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae, |
| author_facet | Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae, |
| author_sort | Wanutsanun Tunyapanit, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Imipenem-resistant Pseudomonas aeruginosa (IRPA) infection is a serious problem in hospitals. Combination therapy is an alternative treatment for this infection. In this study, the in vitro activities of amikacin, aztreonam, ceftazidime, ciprofloxacin, colistin, imipenem, and piperacillin/tazobactam alone and in various combinations were determined by E-test for 38 imipenem-resistant P. aeruginosa isolates obtained from a Thai hospital. Of the 38 IRPA isolates, 9 (24%) were low-level IRPA (defined as MICs of imipenem 8-32 μg/mL) and 29 (76%) were high-level IRPA (defined as MICs of imipenem >32 μg/mL). The high-level IRPA isolates were susceptible to colistin (90%), piperacillin/tazobactam (72%), and amikacin (52%). The low-level IRPA isolates were susceptible to colistin (100%) and all other antimicrobials tested (78%-89%). The MIC50 value of colistin against both the high-level and low-level IRPA isolates was 1.5 μg/mL. Of all the antimicrobial combinations tested, ceftazidime plus ciprofloxacin displayed the highest percentages of synergistic effects against IRPA isolates (26%, 10/38 isolates) and a high percentages of synergistic effects against high-level IRPA isolates (21, 6/29 isolates), with no antagonistic effects detected. Colistin had the greatest activity against most IRPA isolates among all of the antimicrobials tested, while ceftazidime plus ciprofloxacin showed promise in treating infections caused by IRPA isolates including high-level IRPAs. |
| first_indexed | 2025-11-15T00:16:28Z |
| format | Article |
| id | oai:generic.eprints.org:13628 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:16:28Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:136282019-11-10T03:42:29Z http://journalarticle.ukm.my/13628/ In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae, Imipenem-resistant Pseudomonas aeruginosa (IRPA) infection is a serious problem in hospitals. Combination therapy is an alternative treatment for this infection. In this study, the in vitro activities of amikacin, aztreonam, ceftazidime, ciprofloxacin, colistin, imipenem, and piperacillin/tazobactam alone and in various combinations were determined by E-test for 38 imipenem-resistant P. aeruginosa isolates obtained from a Thai hospital. Of the 38 IRPA isolates, 9 (24%) were low-level IRPA (defined as MICs of imipenem 8-32 μg/mL) and 29 (76%) were high-level IRPA (defined as MICs of imipenem >32 μg/mL). The high-level IRPA isolates were susceptible to colistin (90%), piperacillin/tazobactam (72%), and amikacin (52%). The low-level IRPA isolates were susceptible to colistin (100%) and all other antimicrobials tested (78%-89%). The MIC50 value of colistin against both the high-level and low-level IRPA isolates was 1.5 μg/mL. Of all the antimicrobial combinations tested, ceftazidime plus ciprofloxacin displayed the highest percentages of synergistic effects against IRPA isolates (26%, 10/38 isolates) and a high percentages of synergistic effects against high-level IRPA isolates (21, 6/29 isolates), with no antagonistic effects detected. Colistin had the greatest activity against most IRPA isolates among all of the antimicrobials tested, while ceftazidime plus ciprofloxacin showed promise in treating infections caused by IRPA isolates including high-level IRPAs. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13628/1/15%20Wanutsanun%20Tunyapanit.pdf Wanutsanun Tunyapanit, and Pornpimol Pruekprasert, and Kamolwish Laoprasopwattana, and Sureerat Chelae, (2019) In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa. Sains Malaysiana, 48 (5). pp. 1065-1073. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html |
| spellingShingle | Wanutsanun Tunyapanit, Pornpimol Pruekprasert, Kamolwish Laoprasopwattana, Sureerat Chelae, In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa |
| title | In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa |
| title_full | In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa |
| title_fullStr | In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa |
| title_full_unstemmed | In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa |
| title_short | In vitro effects of various antimicrobials alone and in combinations against imipenem-resistant Pseudomonas aeruginosa |
| title_sort | in vitro effects of various antimicrobials alone and in combinations against imipenem-resistant pseudomonas aeruginosa |
| url | http://journalarticle.ukm.my/13628/ http://journalarticle.ukm.my/13628/ http://journalarticle.ukm.my/13628/1/15%20Wanutsanun%20Tunyapanit.pdf |