The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies
The fatty acid analysis in Pliek U and its bioinformatic studies had been carried out and reported. Herein, fatty acids were analyzed by gas chromatography-mass spectrometry (GC-MS), meanwhile their potential effect, based on its interaction on epidermal fatty acid binding protein (E-FABP), was stud...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/13623/ http://journalarticle.ukm.my/13623/1/10%20Nanda%20Earlia.pdf |
| _version_ | 1848813328797794304 |
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| author | Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi |
| author_facet | Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi |
| author_sort | Nanda Earlia, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The fatty acid analysis in Pliek U and its bioinformatic studies had been carried out and reported. Herein, fatty acids were analyzed by gas chromatography-mass spectrometry (GC-MS), meanwhile their potential effect, based on its interaction on epidermal fatty acid binding protein (E-FABP), was studied by bioinformatics approach with reverse docking technique using palmitic acid as a control compound. For the stated purpose, two Pliek U extracts were prepared, namely ethanolic Pliek U extract (EPUE), and ethanolic of residue hexane of Pliek U extract (ERHPUE). The GC-MS results showed that lauric, myristic, palmitic, and oleic acids were predominant, followed by stearic, capric, linoleic, and caprylic acids. Reverse docking results showed that linoleic acid had the lowest binding affinity (-5.9 kcal/mol) and was strongly bound to E-FABP on the same side of amino acid GLN A98, ARG A81, TYR A22, and LYS A61. These findings indicated that linoleic acid has a potential utility as a drug candidate for atopic dermatitis treatment. |
| first_indexed | 2025-11-15T00:16:27Z |
| format | Article |
| id | oai:generic.eprints.org:13623 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:16:27Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:136232019-11-10T04:08:43Z http://journalarticle.ukm.my/13623/ The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi The fatty acid analysis in Pliek U and its bioinformatic studies had been carried out and reported. Herein, fatty acids were analyzed by gas chromatography-mass spectrometry (GC-MS), meanwhile their potential effect, based on its interaction on epidermal fatty acid binding protein (E-FABP), was studied by bioinformatics approach with reverse docking technique using palmitic acid as a control compound. For the stated purpose, two Pliek U extracts were prepared, namely ethanolic Pliek U extract (EPUE), and ethanolic of residue hexane of Pliek U extract (ERHPUE). The GC-MS results showed that lauric, myristic, palmitic, and oleic acids were predominant, followed by stearic, capric, linoleic, and caprylic acids. Reverse docking results showed that linoleic acid had the lowest binding affinity (-5.9 kcal/mol) and was strongly bound to E-FABP on the same side of amino acid GLN A98, ARG A81, TYR A22, and LYS A61. These findings indicated that linoleic acid has a potential utility as a drug candidate for atopic dermatitis treatment. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13623/1/10%20Nanda%20Earlia.pdf Nanda Earlia, and Rahmad, Rahmad and Mohamad Amin, and Prakoeswa, Crs and Khairan, Khairan and Idroes, Rinaldi (2019) The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies. Sains Malaysiana, 48 (5). pp. 1019-1024. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html |
| spellingShingle | Nanda Earlia, Rahmad, Rahmad Mohamad Amin, Prakoeswa, Crs Khairan, Khairan Idroes, Rinaldi The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies |
| title | The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies |
| title_full | The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies |
| title_fullStr | The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies |
| title_full_unstemmed | The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies |
| title_short | The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: chromatography and bioinformatic studies |
| title_sort | potential effect of fatty acids from pliek u on epidermal fatty acid binding protein: chromatography and bioinformatic studies |
| url | http://journalarticle.ukm.my/13623/ http://journalarticle.ukm.my/13623/ http://journalarticle.ukm.my/13623/1/10%20Nanda%20Earlia.pdf |