Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion
Scientists are trying to determine the long term effects of exposure to microgravity and space radiation on various cellular and biological functions. We used C. elegans as the model organism to study the changes in gene expression. Wild type worms were grown in a liquid medium using the CHab hardwa...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Pusat Perubatan Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/13238/ http://journalarticle.ukm.my/13238/1/20-42-1-SM.pdf |
| _version_ | 1848813220708483072 |
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| author | Sue, Mian Then Nurul-Faizah Jusoh, Roslan Harun, Sheila Nathan, Szewczyk, Nathaniel J. Stodieck, Louis Rahman Jamal, |
| author_facet | Sue, Mian Then Nurul-Faizah Jusoh, Roslan Harun, Sheila Nathan, Szewczyk, Nathaniel J. Stodieck, Louis Rahman Jamal, |
| author_sort | Sue, Mian Then |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Scientists are trying to determine the long term effects of exposure to microgravity and space radiation on various cellular and biological functions. We used C. elegans as the model organism to study the changes in gene expression. Wild type worms were grown in a liquid medium using the CHab hardware. The CHab was flown on the STS116 flight to the International Space Station (ISS) and returned to earth on the STS118 flight. Upon landing, surviving worms were extracted and RN Alater added. Ground controls were grown at Bioserve in Colorado and passaged synchronously. RN A was later extracted and mRN A gene expression was analysed using Affymetrix GeneChip® C. elegans Genome Array. The results revealed that 858 known genes were differentially expressed (p-value ≤ 0.05 and fold change ≥ ± 2); namely 608 genes were up-regulated and 250 genes down-regulated. The genes dod-19 and dod-3 which are the downstream effectors of the forkhead transcription factor daf-16 were up-regulated. Daf-16 regulates insulin/TGF signaling pathway that influence metabolic alterations, increased stress and microbial resistance. The glutathione S-transferase (gst-1), Flavin-containing MonoOxygenase (fmo-3) and radiation sensitive genes (rad-51 and him-6) were all up-regulated suggesting responses to oxidative stress. The down-regulation of muscle-related genes (mua-3, col-97, col-109, and col-113) maybe due to reduced mechanical stress in muscle exposed to long-term microgravity. This was the longest exposure of a multi-generational cohort of C. elegans to microgravity which were passaged through at least 10 generations in space. The results suggest key changes in genes involved in ageing, DN A repair, oxidative stress and muscle growth. |
| first_indexed | 2025-11-15T00:14:44Z |
| format | Article |
| id | oai:generic.eprints.org:13238 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:14:44Z |
| publishDate | 2014 |
| publisher | Pusat Perubatan Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:132382019-07-29T04:38:36Z http://journalarticle.ukm.my/13238/ Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion Sue, Mian Then Nurul-Faizah Jusoh, Roslan Harun, Sheila Nathan, Szewczyk, Nathaniel J. Stodieck, Louis Rahman Jamal, Scientists are trying to determine the long term effects of exposure to microgravity and space radiation on various cellular and biological functions. We used C. elegans as the model organism to study the changes in gene expression. Wild type worms were grown in a liquid medium using the CHab hardware. The CHab was flown on the STS116 flight to the International Space Station (ISS) and returned to earth on the STS118 flight. Upon landing, surviving worms were extracted and RN Alater added. Ground controls were grown at Bioserve in Colorado and passaged synchronously. RN A was later extracted and mRN A gene expression was analysed using Affymetrix GeneChip® C. elegans Genome Array. The results revealed that 858 known genes were differentially expressed (p-value ≤ 0.05 and fold change ≥ ± 2); namely 608 genes were up-regulated and 250 genes down-regulated. The genes dod-19 and dod-3 which are the downstream effectors of the forkhead transcription factor daf-16 were up-regulated. Daf-16 regulates insulin/TGF signaling pathway that influence metabolic alterations, increased stress and microbial resistance. The glutathione S-transferase (gst-1), Flavin-containing MonoOxygenase (fmo-3) and radiation sensitive genes (rad-51 and him-6) were all up-regulated suggesting responses to oxidative stress. The down-regulation of muscle-related genes (mua-3, col-97, col-109, and col-113) maybe due to reduced mechanical stress in muscle exposed to long-term microgravity. This was the longest exposure of a multi-generational cohort of C. elegans to microgravity which were passaged through at least 10 generations in space. The results suggest key changes in genes involved in ageing, DN A repair, oxidative stress and muscle growth. Pusat Perubatan Universiti Kebangsaan Malaysia 2014 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13238/1/20-42-1-SM.pdf Sue, Mian Then and Nurul-Faizah Jusoh, and Roslan Harun, and Sheila Nathan, and Szewczyk, Nathaniel J. and Stodieck, Louis and Rahman Jamal, (2014) Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion. Asia-Pacific Journal of Molecular Medicine, 4 (2). pp. 1-11. ISSN 2232-0326 http://spaj.ukm.my/apjmm/index.php/apjmm/issue/view/9 |
| spellingShingle | Sue, Mian Then Nurul-Faizah Jusoh, Roslan Harun, Sheila Nathan, Szewczyk, Nathaniel J. Stodieck, Louis Rahman Jamal, Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion |
| title | Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion |
| title_full | Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion |
| title_fullStr | Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion |
| title_full_unstemmed | Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion |
| title_short | Multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, DNA repair,and locomotion |
| title_sort | multi-generational culture of c. elegans on a long-term space flight revealed changes in expression of genes involved in longevity, dna repair,and locomotion |
| url | http://journalarticle.ukm.my/13238/ http://journalarticle.ukm.my/13238/ http://journalarticle.ukm.my/13238/1/20-42-1-SM.pdf |