Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data
Curcuma aeruginosa or temu hitam is herbaceous plant with high therapeutic values in its rhizome that is widely used in traditional medicine. However, molecular studies on the secondary metabolite biosynthetic pathway of C. aeruginosa is still limited. Hence, the aim of this study was to explore and...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12919/ http://journalarticle.ukm.my/12919/1/07%20Nurul-Syafika%20Mohamad-Fauzi.pdf |
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| author | Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein, |
| author_facet | Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein, |
| author_sort | Nurul-Syafika Mohamad-Fauzi, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Curcuma aeruginosa or temu hitam is herbaceous plant with high therapeutic values in its rhizome that is widely used in traditional medicine. However, molecular studies on the secondary metabolite biosynthetic pathway of C. aeruginosa is still limited. Hence, the aim of this study was to explore and reconstruct the secondary metabolite biosynthetic pathway of C. aeruginosa rhizome by integrating the metabolite profiling and transcriptomic data. A total of 81 metabolites were identified in the rhizome of C. aeruginosa; amongst others are curzerene and β-Cubebene which are potent antioxidants. A total of 28,225 unigene were obtained from the transcriptomic sequencing of C. aeruginosa rhizome and analysed to identify potential genes associated with the biosynthesis of its metabolites. Of these, 43 unigenes were identified and mapped onto five sub-pathways; i.e. carotenoid biosynthetic pathway, diterpenoid biosynthetic pathway, monoterpenoid biosynthetic pathway, terpenoid and steroid biosynthetic pathway, and sesquiterpenoid and triterpenoid biosynthetic pathway. This study demonstrated a systematic bioinformatic approach to reconstruct a metabolic pathway in the rhizome of C. aeruginosa using bioinformatic approach. |
| first_indexed | 2025-11-15T00:13:20Z |
| format | Article |
| id | oai:generic.eprints.org:12919 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:13:20Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:129192019-05-15T11:00:48Z http://journalarticle.ukm.my/12919/ Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein, Curcuma aeruginosa or temu hitam is herbaceous plant with high therapeutic values in its rhizome that is widely used in traditional medicine. However, molecular studies on the secondary metabolite biosynthetic pathway of C. aeruginosa is still limited. Hence, the aim of this study was to explore and reconstruct the secondary metabolite biosynthetic pathway of C. aeruginosa rhizome by integrating the metabolite profiling and transcriptomic data. A total of 81 metabolites were identified in the rhizome of C. aeruginosa; amongst others are curzerene and β-Cubebene which are potent antioxidants. A total of 28,225 unigene were obtained from the transcriptomic sequencing of C. aeruginosa rhizome and analysed to identify potential genes associated with the biosynthesis of its metabolites. Of these, 43 unigenes were identified and mapped onto five sub-pathways; i.e. carotenoid biosynthetic pathway, diterpenoid biosynthetic pathway, monoterpenoid biosynthetic pathway, terpenoid and steroid biosynthetic pathway, and sesquiterpenoid and triterpenoid biosynthetic pathway. This study demonstrated a systematic bioinformatic approach to reconstruct a metabolic pathway in the rhizome of C. aeruginosa using bioinformatic approach. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12919/1/07%20Nurul-Syafika%20Mohamad-Fauzi.pdf Nurul-Syafika Mohamad-Fauzi, and Rabiatul-Adawiah Zainal-Abidin, and Mohd Waznul Adly Zaidan, and Sanimah Simoh, and Alizah Zainal, and Zeti-Azura Mohamed-Hussein, (2018) Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data. Sains Malaysiana, 47 (12). pp. 2985-2992. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html |
| spellingShingle | Nurul-Syafika Mohamad-Fauzi, Rabiatul-Adawiah Zainal-Abidin, Mohd Waznul Adly Zaidan, Sanimah Simoh, Alizah Zainal, Zeti-Azura Mohamed-Hussein, Reconstruction of Curcuma aeruginosa secondary metabolite biosynthetic pathway using omics data |
| title | Reconstruction of Curcuma aeruginosa secondary metabolite
biosynthetic pathway using omics data |
| title_full | Reconstruction of Curcuma aeruginosa secondary metabolite
biosynthetic pathway using omics data |
| title_fullStr | Reconstruction of Curcuma aeruginosa secondary metabolite
biosynthetic pathway using omics data |
| title_full_unstemmed | Reconstruction of Curcuma aeruginosa secondary metabolite
biosynthetic pathway using omics data |
| title_short | Reconstruction of Curcuma aeruginosa secondary metabolite
biosynthetic pathway using omics data |
| title_sort | reconstruction of curcuma aeruginosa secondary metabolite
biosynthetic pathway using omics data |
| url | http://journalarticle.ukm.my/12919/ http://journalarticle.ukm.my/12919/ http://journalarticle.ukm.my/12919/1/07%20Nurul-Syafika%20Mohamad-Fauzi.pdf |