Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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| Online Access: | http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/1/48_01_28.pdf |
| _version_ | 1848813098520018944 |
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| author | Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari, |
| author_facet | Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari, |
| author_sort | Chai, Jun Meng |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in
the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems
with utilization of a growing number of synthetic plastics, PHAs are being considered as a potential substitute for production
of conventional non-degradable plastics. PHAs have been developed as biomaterials with unique properties for many years.
Among all types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is widely sought
after for biomedical application due to the biocompatibility, non-cytotoxicity and non-genotoxicity. Therefore, the aim of this
study is to isolate and identify P(3HB-co-4HB) producers from water and soil sources in Kuala Terengganu. Samples of
lake and soil were collected during November 2017 and then screened for P(3HB-co-4HB) producer. A total of 18 isolates
were obtained, however only 5 isolates were identified as potential PHA producer. Interestingly, 3 isolates were confirmed
as copolymer P(3HB-co-4HB) producer through gas chromatography analysis. These 3 isolated identified bacterial strain
were Cupriavidus sp. TMT 11 with the highest 4HB molar fraction of 14.1%. The other 2 isolates were Acinetobacter sp.
KPD 13 and Cupriavidus sp. PD 16. The properties of the copolymer P(3HB-co-4HB) produced by wide variety of bacteria
isolated can be tailored for various biomedical applications. |
| first_indexed | 2025-11-15T00:12:47Z |
| format | Article |
| id | oai:generic.eprints.org:12797 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:12:47Z |
| publishDate | 2019 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:127972019-04-16T09:11:55Z http://journalarticle.ukm.my/12797/ Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari, Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic plastics, PHAs are being considered as a potential substitute for production of conventional non-degradable plastics. PHAs have been developed as biomaterials with unique properties for many years. Among all types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is widely sought after for biomedical application due to the biocompatibility, non-cytotoxicity and non-genotoxicity. Therefore, the aim of this study is to isolate and identify P(3HB-co-4HB) producers from water and soil sources in Kuala Terengganu. Samples of lake and soil were collected during November 2017 and then screened for P(3HB-co-4HB) producer. A total of 18 isolates were obtained, however only 5 isolates were identified as potential PHA producer. Interestingly, 3 isolates were confirmed as copolymer P(3HB-co-4HB) producer through gas chromatography analysis. These 3 isolated identified bacterial strain were Cupriavidus sp. TMT 11 with the highest 4HB molar fraction of 14.1%. The other 2 isolates were Acinetobacter sp. KPD 13 and Cupriavidus sp. PD 16. The properties of the copolymer P(3HB-co-4HB) produced by wide variety of bacteria isolated can be tailored for various biomedical applications. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12797/1/48_01_28.pdf Chai, Jun Meng and Manogiri Sadasivam, and Sevakumaran Vigneswari, (2019) Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu. Malaysian Applied Biology, 48 (1). pp. 199-206. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56 |
| spellingShingle | Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari, Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu |
| title | Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu |
| title_full | Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu |
| title_fullStr | Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu |
| title_full_unstemmed | Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu |
| title_short | Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu |
| title_sort | isolation and identification of p(3hb-co-4hb) producing bacteria from various locations in kuala terengganu |
| url | http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/ http://journalarticle.ukm.my/12797/1/48_01_28.pdf |