Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa

Microbial cellulose (MC) is an alternative source of cellulose that can be produced by different types of microorganisms such as Pseudomonas, Komagataeibacter, Sarcina and Azotobacter and they are commonly used in biomedical applications. However, the main issue in MC production is low yield. To e...

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Main Authors: Kang, Wern Ni, Azila Adnan
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/12769/
http://journalarticle.ukm.my/12769/1/48_01_02.pdf
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author Kang, Wern Ni
Azila Adnan,
author_facet Kang, Wern Ni
Azila Adnan,
author_sort Kang, Wern Ni
building UKM Institutional Repository
collection Online Access
description Microbial cellulose (MC) is an alternative source of cellulose that can be produced by different types of microorganisms such as Pseudomonas, Komagataeibacter, Sarcina and Azotobacter and they are commonly used in biomedical applications. However, the main issue in MC production is low yield. To enhance MC production, different combinations of the dualsaccharides, such as 25 g/L glucose + 25 g/L galactose, 25 g/L glucose + 25 g/L fructose, 25 g/L glucose + 25 g/L maltose and 25 g/L glucose + 25 g/L lactose were added into culture medium. The fermentation was carried out at 30oC for 5 days in incubator shaker. The combination of glucose and galactose (1:1) gave a higher concentration of MC with 1.23 g/L ± 0.15. However, it was lower than glucose (control) (1.8 g/L ± 0.63). The glucose + galactose concentrations varied between 10 g/ L, 30 g/L, 50 g/L and 70 g/L for the MC production. Therefore, the concentration of 50 g/L is the best for the the MC production. Increasing the concentration of glucose + galactose beyond 50 g/L decreased MC production.
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spelling oai:generic.eprints.org:127692019-04-16T08:55:09Z http://journalarticle.ukm.my/12769/ Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa Kang, Wern Ni Azila Adnan, Microbial cellulose (MC) is an alternative source of cellulose that can be produced by different types of microorganisms such as Pseudomonas, Komagataeibacter, Sarcina and Azotobacter and they are commonly used in biomedical applications. However, the main issue in MC production is low yield. To enhance MC production, different combinations of the dualsaccharides, such as 25 g/L glucose + 25 g/L galactose, 25 g/L glucose + 25 g/L fructose, 25 g/L glucose + 25 g/L maltose and 25 g/L glucose + 25 g/L lactose were added into culture medium. The fermentation was carried out at 30oC for 5 days in incubator shaker. The combination of glucose and galactose (1:1) gave a higher concentration of MC with 1.23 g/L ± 0.15. However, it was lower than glucose (control) (1.8 g/L ± 0.63). The glucose + galactose concentrations varied between 10 g/ L, 30 g/L, 50 g/L and 70 g/L for the MC production. Therefore, the concentration of 50 g/L is the best for the the MC production. Increasing the concentration of glucose + galactose beyond 50 g/L decreased MC production. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12769/1/48_01_02.pdf Kang, Wern Ni and Azila Adnan, (2019) Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa. Malaysian Applied Biology, 48 (1). pp. 7-14. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
spellingShingle Kang, Wern Ni
Azila Adnan,
Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_full Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_fullStr Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_full_unstemmed Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_short Effect of dual-saccharides on microbial cellulose (MC) production by Pseudomonas aeruginosa
title_sort effect of dual-saccharides on microbial cellulose (mc) production by pseudomonas aeruginosa
url http://journalarticle.ukm.my/12769/
http://journalarticle.ukm.my/12769/
http://journalarticle.ukm.my/12769/1/48_01_02.pdf