Sensitivity evaluation of SYBR green I, SYBR safe and calcein dyes for detection of human papillomavirus 16 by loop-mediated isothermal amplification

QR Code

Sensitivity evaluation of SYBR green I, SYBR safe and calcein dyes for detection of human papillomavirus 16 by loop-mediated isothermal amplification

Loop-mediated isothermal amplification (LAMP) is a gene amplification technique whereby the amplification products are commonly visualized as turbidity by naked eye in the presence of magnesium pyrophosphate precipitation. An appropriate intercalating dye is important as it could increase the sensit...

Full description

Bibliographic Details
Main Authors:	Suharni Mohamad, Nuur Ezzatyhusna Mk, Nurul Izzati Hassan, Siti Suraiya MN, Suharni M
Format:	Article
Language:	English
Published:	Pusat Perubatan Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12682/ http://journalarticle.ukm.my/12682/1/7_nuur_et_al_pdf_56028.pdf

Similar Items

Development of real-time loopmediated isothermal amplification and PCR assays for detection of human papillomavirus 16 in oral squamous cell carcinoma
by: Hamzan, Nurul Izzati
Published: (2020)

Optimization of qPCR assay of chicken cytokine genes using sybr green method
by: Chan, Jia Hui
Published: (2015)

Development of a sybr green based real-time polymerase chain reaction assay for specific detection and quantification of vibrio parahaemolyticus from selected seafood Key words: Vibrio parahaemolyticus, real-time polymerase chain reaction (RT-PCR), SYBR Green dye, seafood
by: Nur Quraitu' Aini, Binti Tajudin
Published: (2012)

Development of loop-mediated isothermal amplification technique for detection of Candida glabrata
by: Hassan, Yahaya
Published: (2020)

Loop-mediated isothermal amplification for the detection of infectious spleen and kidney necrosis virus
by: Mohamed Din, Mohamed Shariff, et al.
Published: (2013)

Development of reverse transcription loop-mediated isothermal amplification for detection of Rhinovirus C
by: Wong, Yien Ping
Published: (2019)

Sybr green real-time PCR is a useful method in screening for high risk human papilloma virus types 16 and 18 in neoplastic cervical cancer
by: Nor Rizan K,, et al.
Published: (2011)

Detection of Newcastle disease virus using a SYBR Green I real time polymerase chain reaction
by: Tan, Sheau Wei, et al.
Published: (2004)

Development Of Sybr Green I Real-Time Pcr Method For Detection And Differentiation Of Newcastle Disease Virus Pathotypes
by: Tan, Sheau Wei
Published: (2008)

Rapid detection of Newcastle disease virus (NDV) with SYBR Green I real-time polymerase chain reaction
by: Tan, Sheau Wei, et al.
Published: (2004)

Detection of infectious bursal disease virus using SYBR Green 1 based realtime polymerase chain reaction
by: Hamzah, Hairul Aini, et al.
Published: (2004)

Investigation of boris and ctcf genes expression by sybr-green based realtime rt-pcr in human brain tumors
by: Faizul Rahman, Sjafri
Published: (2008)

Loop‐mediated isothermal amplification (LAMP): a versatile technique for detection of micro‐organisms
by: Wong, YP, et al.
Published: (2018)

Comparison of Sybr Green I, ELISA and conventional agarose gel-based PCR in the detection of infectious bursal disease virus
by: Hamzah, Hairul Aini, et al.
Published: (2008)

The investigation of cyclin-di gene expression using sybr green 1 based real-time pcr in bladder cancer
by: Aisya, Zulkifli
Published: (2009)

Design, optimization, fabrication and analysis of Cu microheater for loop-mediated isothermal amplification (LAMP) applications
by: Ezhilnavaroji, Jeroish Zachariah, et al.
Published: (2023)

Room-temperature stable loop-mediated isothermal amplification LAMP reagents to detect leptospiral DNA
by: Lee, Pui Yuei, et al.
Published: (2021)

Detection of leptospiral DNA in rat kidney and environmental samples using loop-mediated isothermal amplification
by: Lee, Pui Yuei
Published: (2019)

Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection
by: Yahaya, H., et al.
Published: (2024)

Development of reverse transcription loop-mediated isothermal amplification for rapid detection of Sars-Cov-2
by: Attah, Obande Godwin
Published: (2022)

Development of Sybr Green 1 Based Real-Time Polymerase Chain Reaction for Detection and Differentiation of Infectious Bursal Disease Virus
by: Hamzah, Hairul Aini
Published: (2005)

Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of toxigenic vibrio cholerae
by: Engku Abd Rahman , Engku Nur Syafirah
Published: (2017)

Development of loop-mediated isothermal amplification for a simple and rapid detection of leptospiral DNA in human samples
by: Othman, Shuhaidah
Published: (2018)

Loop-mediated isothermal amplification (LAMP): comparative advances over conventional PCR and other molecular techniques
by: Hassan, Yahaya, et al.
Published: (2020)

The evaluation of loop mediated isothermal amplification (LAMP) for the detection of porphyromonas gingivalis using heat-treated saliva
by: Hanan, Rosnani Che AWang @ Abdul
Published: (2014)

Molecular identification of species and production origins of edible bird's nest using FINS and SYBR green I based real-time PCR
by: Meei, Chien Quek, et al.
Published: (2018)

Investigation of CTCF gene in Glioma and its correlation with YB-1 gene using SYBR-Green I based real-time PCR
by: Kahar, Nazirah Abd
Published: (2009)

Sensitive detection of PIK3CA Exon 20 H1047R breast cancer based on lowcost intercalary dye SYBR Green I real-time qPCR assay
by: Desriani,, et al.
Published: (2024)

Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research
by: Padzil, Faiz, et al.
Published: (2021)

Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers
by: Tan, Sheau Wei, et al.
Published: (2009)

Use of acridine orange to visually improve the loop-mediated isothermal amplification for detection of infectious spleen and kidney necrosis virus
by: Subramaniam, Kuttichantran, et al.
Published: (2014)

Detection of Coconut cadang-cadang viroid (CCCVd) in oil palm by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
by: Thanarajoo, Sathis Sri, et al.
Published: (2014)

Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products
by: Tasrip, Nor Asmara, et al.
Published: (2019)

Detection of coconut cadang-cadang viroid variants (CCCVd) in coconut by reverse transcription loop-mediated isothermal amplification (RT-LAMP)
by: Mat Husain, Siti Rohani
Published: (2015)

Electrochemical biosensor based on silicon nanowires/ platinum nanoparticles associated with loop-mediated isothermal amplification for detection of porcine DNA in food
by: Kusnin, Norzila
Published: (2024)

Development of a SYBR green based real-time polymerase chain reaction assay for specific detection and quantification of Vibrio parahaemolyticus from food and environmental samples.
by: V, Micky, et al.
Published: (2014)

A loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of airborne inoculum of Uromyces betae (sugar beet rust)
by: Kaczmarek, Agata Monika, et al.
Published: (2018)

Development of SYBR green I based one-step real-time RT-PCR assay for the detection and differentiation of very virulent and classical strains of infectious bursal disease virus
by: Lih, Ling Kong, et al.
Published: (2009)

Development of real-time fluorescence multiplex loop-mediated isothermal amplification assay for simultaneous detection of Streptococcus pneumoniae and Haemophilus influenzae / Nee Yuan Qi
by: Nee , Yuan Qi
Published: (2018)

Production Of Uvrd Helicase And Mutl Recombinant Proteins For Helicasedependent Isothermal Amplification
by: Sepuloh Maniam, Sanggetha Periya
Published: (2015)