Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations
Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active alkaloids of R. angustifolia and evaluating their potential as synergistic enhancers in alkalo...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12512/ http://journalarticle.ukm.my/12512/1/19%20Laina%20Zarisa%20Mohd%20Kamal.pdf |
| _version_ | 1848813022831706112 |
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| author | Laina Zarisa Mohd Kamal, Norazian Mohd Hassan, Nurhaya Md Taib, May, Khin Soe |
| author_facet | Laina Zarisa Mohd Kamal, Norazian Mohd Hassan, Nurhaya Md Taib, May, Khin Soe |
| author_sort | Laina Zarisa Mohd Kamal, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active alkaloids of R. angustifolia and evaluating their potential as synergistic enhancers in alkaloid-antibiotic combinations. Antimicrobial bioautography-guided isolation of alkaloidal fractions of R. angustifolia leaves has led to the identification of 2,3-dimethoxy-1-hydroxy-10-methylacridone [arborinine]; and 4,7,8-trimethoxyfuro[2,3-b]quinoline [skimmianine]; together with the major active alkaloid, 1-methyl-2-[3’,4’-methylenedioxyphenyl]-4-quinolone [graveoline]. Graveoline showed Minimum Inhibitory Concentration (MIC) values ranging from 500 to 1000 μg/mL against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 and Escherichia coli ATCC 25922. Checkerboard assay for antimicrobial combination effects between graveoline with either erythromycin or vancomycin showed enhancement of the antimicrobial activity of both antibiotics with Fractional Inhibitory Concentration Indices (FICI) ranged from 0.37 to 1.50. Synergistic effect with FICI of 0.37 was observed for graveoline-erythromycin combination against S. aureus compared to FICI of 1.00 for ciprofloxacin-erythromycin additive effect. Graveoline was a potential candidate for antimicrobial combination agent especially against S. aureus. The result supports the idea of using plant metabolites as antimicrobial synergistic agents. |
| first_indexed | 2025-11-15T00:11:35Z |
| format | Article |
| id | oai:generic.eprints.org:12512 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:11:35Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:125122019-01-28T21:24:57Z http://journalarticle.ukm.my/12512/ Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations Laina Zarisa Mohd Kamal, Norazian Mohd Hassan, Nurhaya Md Taib, May, Khin Soe Ruta angustifolia (L.) Pers. is a Rutaceous species which contains various anthranilic acid derived alkaloids including the bioactive quinolones. This study is aimed at identifying the antimicrobial active alkaloids of R. angustifolia and evaluating their potential as synergistic enhancers in alkaloid-antibiotic combinations. Antimicrobial bioautography-guided isolation of alkaloidal fractions of R. angustifolia leaves has led to the identification of 2,3-dimethoxy-1-hydroxy-10-methylacridone [arborinine]; and 4,7,8-trimethoxyfuro[2,3-b]quinoline [skimmianine]; together with the major active alkaloid, 1-methyl-2-[3’,4’-methylenedioxyphenyl]-4-quinolone [graveoline]. Graveoline showed Minimum Inhibitory Concentration (MIC) values ranging from 500 to 1000 μg/mL against Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212 and Escherichia coli ATCC 25922. Checkerboard assay for antimicrobial combination effects between graveoline with either erythromycin or vancomycin showed enhancement of the antimicrobial activity of both antibiotics with Fractional Inhibitory Concentration Indices (FICI) ranged from 0.37 to 1.50. Synergistic effect with FICI of 0.37 was observed for graveoline-erythromycin combination against S. aureus compared to FICI of 1.00 for ciprofloxacin-erythromycin additive effect. Graveoline was a potential candidate for antimicrobial combination agent especially against S. aureus. The result supports the idea of using plant metabolites as antimicrobial synergistic agents. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12512/1/19%20Laina%20Zarisa%20Mohd%20Kamal.pdf Laina Zarisa Mohd Kamal, and Norazian Mohd Hassan, and Nurhaya Md Taib, and May, Khin Soe (2018) Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations. Sains Malaysiana, 47 (10). pp. 2429-2435. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil10_2018/KandunganJilid47Bil10_2018.htm |
| spellingShingle | Laina Zarisa Mohd Kamal, Norazian Mohd Hassan, Nurhaya Md Taib, May, Khin Soe Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations |
| title | Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations |
| title_full | Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations |
| title_fullStr | Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations |
| title_full_unstemmed | Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations |
| title_short | Graveoline from Ruta angustifolia (L.) Pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations |
| title_sort | graveoline from ruta angustifolia (l.) pers. and its antimicrobial synergistic potential in erythromycin or vancomycin combinations |
| url | http://journalarticle.ukm.my/12512/ http://journalarticle.ukm.my/12512/ http://journalarticle.ukm.my/12512/1/19%20Laina%20Zarisa%20Mohd%20Kamal.pdf |