Characterisation of recombinant Trichoderma reesei cellobiohydrolase and the potential of cellulase mixture in hydrolyzing oil palm empty fruit bunches
The potential for recombinant cellulases from fungi to hydrolyze oil palm empty fruit bunches (OPEFB) into simple sugars was investigated. A mixture of two recombinant enzymes consisting of a cellobiohydrolase from Trichoderma reesei and an endoglucanase from Aspergillus niger, was evaluated for O...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12327/ http://journalarticle.ukm.my/12327/1/46_02_02.pdf |
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| author | Quay, Doris Huai Xia Yong, Hui Yee Rosli Md Illias, Nor Muhammad Mahadi, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, |
| author_facet | Quay, Doris Huai Xia Yong, Hui Yee Rosli Md Illias, Nor Muhammad Mahadi, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, |
| author_sort | Quay, Doris Huai Xia |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The potential for recombinant cellulases from fungi to hydrolyze oil palm empty fruit bunches (OPEFB) into simple sugars
was investigated. A mixture of two recombinant enzymes consisting of a cellobiohydrolase from Trichoderma reesei and an
endoglucanase from Aspergillus niger, was evaluated for OPEFB hydrolysis. The development of A. niger endoglucanase
has been described previously. In this work, the development of a recombinant cellobiohydrolase and the activity of this
enzyme mixture towards OPEFB hydrolysis were described. To obtain recombinant cellobiohydrolase, the cDNA encoding
for cellobiohydrolase, cbhII, was isolated from the T. reesei strain M5, and the gene was expressed in the methylotrophic
yeast, Pichia pastoris. Partially purified CbhII demonstrated optimum activity at 50°C and pH 5.0. This enzyme was shown
to hydrolyze Avicel at a concentration of 0.486 U/mg under optimum conditions. Hydrolysis of pretreated OPEFB using
CbhII and endoglucanase EglA showed that the total reducing sugar produced was higher in the enzyme mixture reaction
than were produced in an individual enzyme reaction. Addition of β-glucosidase and an enzyme cofactor significantly increased
enzyme activity and OPEFB cellulose hydrolysis. This study demonstrated the capability of an enzyme mixture to produce
sugars from oil palm lignocellulosic waste. |
| first_indexed | 2025-11-15T00:10:46Z |
| format | Article |
| id | oai:generic.eprints.org:12327 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:10:46Z |
| publishDate | 2017 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:123272018-11-16T21:10:58Z http://journalarticle.ukm.my/12327/ Characterisation of recombinant Trichoderma reesei cellobiohydrolase and the potential of cellulase mixture in hydrolyzing oil palm empty fruit bunches Quay, Doris Huai Xia Yong, Hui Yee Rosli Md Illias, Nor Muhammad Mahadi, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, The potential for recombinant cellulases from fungi to hydrolyze oil palm empty fruit bunches (OPEFB) into simple sugars was investigated. A mixture of two recombinant enzymes consisting of a cellobiohydrolase from Trichoderma reesei and an endoglucanase from Aspergillus niger, was evaluated for OPEFB hydrolysis. The development of A. niger endoglucanase has been described previously. In this work, the development of a recombinant cellobiohydrolase and the activity of this enzyme mixture towards OPEFB hydrolysis were described. To obtain recombinant cellobiohydrolase, the cDNA encoding for cellobiohydrolase, cbhII, was isolated from the T. reesei strain M5, and the gene was expressed in the methylotrophic yeast, Pichia pastoris. Partially purified CbhII demonstrated optimum activity at 50°C and pH 5.0. This enzyme was shown to hydrolyze Avicel at a concentration of 0.486 U/mg under optimum conditions. Hydrolysis of pretreated OPEFB using CbhII and endoglucanase EglA showed that the total reducing sugar produced was higher in the enzyme mixture reaction than were produced in an individual enzyme reaction. Addition of β-glucosidase and an enzyme cofactor significantly increased enzyme activity and OPEFB cellulose hydrolysis. This study demonstrated the capability of an enzyme mixture to produce sugars from oil palm lignocellulosic waste. Penerbit Universiti Kebangsaan Malaysia 2017-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12327/1/46_02_02.pdf Quay, Doris Huai Xia and Yong, Hui Yee and Rosli Md Illias, and Nor Muhammad Mahadi, and Farah Diba Abu Bakar, and Abdul Munir Abdul Murad, (2017) Characterisation of recombinant Trichoderma reesei cellobiohydrolase and the potential of cellulase mixture in hydrolyzing oil palm empty fruit bunches. Malaysian Applied Biology, 46 (2). pp. 11-19. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=644&catid=59:current-view&Itemid=56 |
| spellingShingle | Quay, Doris Huai Xia Yong, Hui Yee Rosli Md Illias, Nor Muhammad Mahadi, Farah Diba Abu Bakar, Abdul Munir Abdul Murad, Characterisation of recombinant Trichoderma reesei cellobiohydrolase and the potential of cellulase mixture in hydrolyzing oil palm empty fruit bunches |
| title | Characterisation of recombinant Trichoderma reesei
cellobiohydrolase and the potential of
cellulase mixture in hydrolyzing oil palm
empty fruit bunches |
| title_full | Characterisation of recombinant Trichoderma reesei
cellobiohydrolase and the potential of
cellulase mixture in hydrolyzing oil palm
empty fruit bunches |
| title_fullStr | Characterisation of recombinant Trichoderma reesei
cellobiohydrolase and the potential of
cellulase mixture in hydrolyzing oil palm
empty fruit bunches |
| title_full_unstemmed | Characterisation of recombinant Trichoderma reesei
cellobiohydrolase and the potential of
cellulase mixture in hydrolyzing oil palm
empty fruit bunches |
| title_short | Characterisation of recombinant Trichoderma reesei
cellobiohydrolase and the potential of
cellulase mixture in hydrolyzing oil palm
empty fruit bunches |
| title_sort | characterisation of recombinant trichoderma reesei
cellobiohydrolase and the potential of
cellulase mixture in hydrolyzing oil palm
empty fruit bunches |
| url | http://journalarticle.ukm.my/12327/ http://journalarticle.ukm.my/12327/ http://journalarticle.ukm.my/12327/1/46_02_02.pdf |