Immobilization of bovine serum albumin on the chitosan/PVA film

Chitosan/polyvinyl alcohol (Chitosan/PVA) blended film was prepared by direct blend process and solution casting methods. In order to reduce the swelling ratio and enhance the chemical and mechanical stability, Chitosan/PVA film was cross-linked with glutaraldehyde in order to produce Chitosan-g-PVA...

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Main Authors: Nurul Mujahidah Ahmad Khairuddin, Amalina Muhammad Afifi, Nur Awanis Hashim, Shaza Eva Mohamad, Katayoon Kalantari
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12137/
http://journalarticle.ukm.my/12137/1/28%20Nurul%20Mujahidah%20Ahmad%20Khairuddin.pdf
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author Nurul Mujahidah Ahmad Khairuddin,
Amalina Muhammad Afifi,
Nur Awanis Hashim,
Shaza Eva Mohamad,
Katayoon Kalantari,
author_facet Nurul Mujahidah Ahmad Khairuddin,
Amalina Muhammad Afifi,
Nur Awanis Hashim,
Shaza Eva Mohamad,
Katayoon Kalantari,
author_sort Nurul Mujahidah Ahmad Khairuddin,
building UKM Institutional Repository
collection Online Access
description Chitosan/polyvinyl alcohol (Chitosan/PVA) blended film was prepared by direct blend process and solution casting methods. In order to reduce the swelling ratio and enhance the chemical and mechanical stability, Chitosan/PVA film was cross-linked with glutaraldehyde in order to produce Chitosan-g-PVA. Bovine serum albumin (BSA) was used as a model protein to incorporate into the Chitosan-g-PVA. The chemical structure and morphological characteristics of films were studied by FT-IR and scanning electron microscopy (SEM). Mechanical and physical properties of blended films such as tensile properties in the dry and wet states, water uptake and water contact angle measurement were characterized. Blending PVA and chitosan improved strength and flexibility of the films. Crosslinking with glutaraldehyde further improves the tensile strength and decrease the hydrophilicity of films. BSA immobilized on the Chitosan-g-PVA film was calculated as BSA encapsulation efficiency.
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publishDate 2018
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spelling oai:generic.eprints.org:121372018-09-28T22:45:27Z http://journalarticle.ukm.my/12137/ Immobilization of bovine serum albumin on the chitosan/PVA film Nurul Mujahidah Ahmad Khairuddin, Amalina Muhammad Afifi, Nur Awanis Hashim, Shaza Eva Mohamad, Katayoon Kalantari, Chitosan/polyvinyl alcohol (Chitosan/PVA) blended film was prepared by direct blend process and solution casting methods. In order to reduce the swelling ratio and enhance the chemical and mechanical stability, Chitosan/PVA film was cross-linked with glutaraldehyde in order to produce Chitosan-g-PVA. Bovine serum albumin (BSA) was used as a model protein to incorporate into the Chitosan-g-PVA. The chemical structure and morphological characteristics of films were studied by FT-IR and scanning electron microscopy (SEM). Mechanical and physical properties of blended films such as tensile properties in the dry and wet states, water uptake and water contact angle measurement were characterized. Blending PVA and chitosan improved strength and flexibility of the films. Crosslinking with glutaraldehyde further improves the tensile strength and decrease the hydrophilicity of films. BSA immobilized on the Chitosan-g-PVA film was calculated as BSA encapsulation efficiency. Penerbit Universiti Kebangsaan Malaysia 2018-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12137/1/28%20Nurul%20Mujahidah%20Ahmad%20Khairuddin.pdf Nurul Mujahidah Ahmad Khairuddin, and Amalina Muhammad Afifi, and Nur Awanis Hashim, and Shaza Eva Mohamad, and Katayoon Kalantari, (2018) Immobilization of bovine serum albumin on the chitosan/PVA film. Sains Malaysiana, 47 (6). pp. 1311-1318. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num6_2018/contentsVol47num6_2018.html
spellingShingle Nurul Mujahidah Ahmad Khairuddin,
Amalina Muhammad Afifi,
Nur Awanis Hashim,
Shaza Eva Mohamad,
Katayoon Kalantari,
Immobilization of bovine serum albumin on the chitosan/PVA film
title Immobilization of bovine serum albumin on the chitosan/PVA film
title_full Immobilization of bovine serum albumin on the chitosan/PVA film
title_fullStr Immobilization of bovine serum albumin on the chitosan/PVA film
title_full_unstemmed Immobilization of bovine serum albumin on the chitosan/PVA film
title_short Immobilization of bovine serum albumin on the chitosan/PVA film
title_sort immobilization of bovine serum albumin on the chitosan/pva film
url http://journalarticle.ukm.my/12137/
http://journalarticle.ukm.my/12137/
http://journalarticle.ukm.my/12137/1/28%20Nurul%20Mujahidah%20Ahmad%20Khairuddin.pdf