Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana
The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems a...
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| Format: | Article |
| Language: | English |
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Penebit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/1/09%20Gan%20Khai%20Swan.pdf |
| _version_ | 1848812891122171904 |
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| author | Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna, |
| author_facet | Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna, |
| author_sort | Gan, Khai Swan |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use. |
| first_indexed | 2025-11-15T00:09:30Z |
| format | Article |
| id | oai:generic.eprints.org:12031 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:09:30Z |
| publishDate | 2018 |
| publisher | Penebit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:120312018-09-09T23:17:51Z http://journalarticle.ukm.my/12031/ Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna, The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use. Penebit Universiti Kebangsaan Malaysia 2018-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12031/1/09%20Gan%20Khai%20Swan.pdf Gan, Khai Swan and Tan, Chon Seng and Rofina Yasmin Othman, and Jennifer Ann Harikrishna, (2018) Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana. Sains Malaysiana, 47 (3). pp. 499-509. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil3_2018/KandunganJilid47Bil3_2018.html |
| spellingShingle | Gan, Khai Swan Tan, Chon Seng Rofina Yasmin Othman, Jennifer Ann Harikrishna, Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana |
| title | Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana |
| title_full | Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana |
| title_fullStr | Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana |
| title_full_unstemmed | Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana |
| title_short | Transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in Nicotiana benthamiana |
| title_sort | transient expression of an immunogenic envelope attachment glycoprotein of nipah virus in nicotiana benthamiana |
| url | http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/ http://journalarticle.ukm.my/12031/1/09%20Gan%20Khai%20Swan.pdf |