Optimization of illumina Truseq Targeted RNA expression (TREx) library quality
RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimiza...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2018
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| Online Access: | http://journalarticle.ukm.my/12009/ http://journalarticle.ukm.my/12009/1/UKM%20SAINSMalaysiana%2047%2802%29Feb%202018%20%2012.pdf |
| _version_ | 1848812885023653888 |
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| author | Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen, |
| author_facet | Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen, |
| author_sort | Nadiah Abu, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and
technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In
this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell
line. Total RNA was recommended as starting materials but it required optimization including additional purification
step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyAenriched
mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be
prepared by adding multiple washing steps or changing input sample to mRNA. |
| first_indexed | 2025-11-15T00:09:24Z |
| format | Article |
| id | oai:generic.eprints.org:12009 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:09:24Z |
| publishDate | 2018 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:120092018-08-21T07:14:49Z http://journalarticle.ukm.my/12009/ Optimization of illumina Truseq Targeted RNA expression (TREx) library quality Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen, RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell line. Total RNA was recommended as starting materials but it required optimization including additional purification step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyAenriched mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be prepared by adding multiple washing steps or changing input sample to mRNA. Penerbit Universiti Kebangsaan Malaysia 2018-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12009/1/UKM%20SAINSMalaysiana%2047%2802%29Feb%202018%20%2012.pdf Nadiah Abu, and Yeap, Swee Keong and Noraini Nordin, and Tan, Sheau Wei and Ho, Wan Yong and Noorjahan Banu Alitheen, (2018) Optimization of illumina Truseq Targeted RNA expression (TREx) library quality. Sains Malaysiana, 47 (2). pp. 303-308. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num2_2018/contentsVol47num2_2018.html |
| spellingShingle | Nadiah Abu, Yeap, Swee Keong Noraini Nordin, Tan, Sheau Wei Ho, Wan Yong Noorjahan Banu Alitheen, Optimization of illumina Truseq Targeted RNA expression (TREx) library quality |
| title | Optimization of illumina Truseq Targeted RNA expression (TREx) library quality |
| title_full | Optimization of illumina Truseq Targeted RNA expression (TREx) library quality |
| title_fullStr | Optimization of illumina Truseq Targeted RNA expression (TREx) library quality |
| title_full_unstemmed | Optimization of illumina Truseq Targeted RNA expression (TREx) library quality |
| title_short | Optimization of illumina Truseq Targeted RNA expression (TREx) library quality |
| title_sort | optimization of illumina truseq targeted rna expression (trex) library quality |
| url | http://journalarticle.ukm.my/12009/ http://journalarticle.ukm.my/12009/ http://journalarticle.ukm.my/12009/1/UKM%20SAINSMalaysiana%2047%2802%29Feb%202018%20%2012.pdf |