Cloning, heterologous expression and characterisation of a recombinant cellobiohydrolase from Humicola insolens ATCC16454 in Pichia pastoris

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Cloning, heterologous expression and characterisation of a recombinant cellobiohydrolase from Humicola insolens ATCC16454 in Pichia pastoris

A cellobiohydrolase gene from the thermophilic fungus Humicola insolens ATCC 16454 was expressed in the methylotrophic yeast Pichia pastoris X-33, and the biochemical properties of the recombinant protein were characterised. The full-length cDNA of the cellobiohydrolase gene avi2 was cloned into t...

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Bibliographic Details
Main Authors:	Amatul Samahah Md Ali, Farah Diba Abu Bakar, Rosli Md Illias, Osman Hassan, Abdul Munir Abdul Murad
Format:	Article
Language:	English
Published:	Malaysian Society of Applied Biology 2015
Online Access:	http://journalarticle.ukm.my/11786/ http://journalarticle.ukm.my/11786/1/44_4_03.pdf

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