A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen
A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV). Ag NPs were initially deposited onto amine functionalized glass slides. Subsequently, JEV antibodies were self-assembled onto surfaces of Ag NPs to form optica...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/11713/ http://journalarticle.ukm.my/11713/1/21%20Lih%20Shan%20Lim.pdf |
| _version_ | 1848812806029180928 |
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| author | Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera, |
| author_facet | Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera, |
| author_sort | Lim, Lih Shan |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV). Ag NPs were initially deposited onto amine functionalized glass slides. Subsequently, JEV antibodies were self-assembled onto surfaces of Ag NPs to form optical sensing probes. The detection of JEV antigen was observed via changes in light absorbance by Ag NPs upon occurrence of JEV antigen-antibody bindings. A highly sensitive and rapid optical sensing probe for JEV antigen with a detection limit of 12.8 ng/mL (for S/N ratio = 3) and an analysis assay time of 1 h had been demonstrated. |
| first_indexed | 2025-11-15T00:08:08Z |
| format | Article |
| id | oai:generic.eprints.org:11713 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:08:08Z |
| publishDate | 2017 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:117132018-05-30T00:52:21Z http://journalarticle.ukm.my/11713/ A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera, A novel silver nanoparticles (Ag NPs)-based optical sensing probe has been developed for the detection of Japanese Encephalitis virus (JEV). Ag NPs were initially deposited onto amine functionalized glass slides. Subsequently, JEV antibodies were self-assembled onto surfaces of Ag NPs to form optical sensing probes. The detection of JEV antigen was observed via changes in light absorbance by Ag NPs upon occurrence of JEV antigen-antibody bindings. A highly sensitive and rapid optical sensing probe for JEV antigen with a detection limit of 12.8 ng/mL (for S/N ratio = 3) and an analysis assay time of 1 h had been demonstrated. Penerbit Universiti Kebangsaan Malaysia 2017-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11713/1/21%20Lih%20Shan%20Lim.pdf Lim, Lih Shan and Chin, Suk Fun and Pang, Suh Cem and Magdline Sia Henry Sum, and David Perera, (2017) A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen. Sains Malaysiana, 46 (12). pp. 2447-2454. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num12_2017/contentsVol46num12_2017.htm |
| spellingShingle | Lim, Lih Shan Chin, Suk Fun Pang, Suh Cem Magdline Sia Henry Sum, David Perera, A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen |
| title | A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen |
| title_full | A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen |
| title_fullStr | A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen |
| title_full_unstemmed | A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen |
| title_short | A novel silver nanoparticles-based sensing probe for the detection of Japanese Encephalitis virus antigen |
| title_sort | novel silver nanoparticles-based sensing probe for the detection of japanese encephalitis virus antigen |
| url | http://journalarticle.ukm.my/11713/ http://journalarticle.ukm.my/11713/ http://journalarticle.ukm.my/11713/1/21%20Lih%20Shan%20Lim.pdf |