Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts
Quassia borneensis has been traditionally used as antihypertensive agent without any scientific literature on its mechanism of action. The objective of this study was to evaluate the antiinflammatory, antioxidant and antiproliferation properties of Q. borneensis extracts. The hexane, chloroform and...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/11456/ http://journalarticle.ukm.my/11456/1/16743-47128-1-SM.pdf |
| _version_ | 1848812732001812480 |
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| author | Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali, |
| author_facet | Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali, |
| author_sort | Firdaus Kamarulzaman, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Quassia borneensis has been traditionally used as antihypertensive agent without any scientific literature on its mechanism of action. The objective of this study was to evaluate the antiinflammatory, antioxidant and antiproliferation properties of Q. borneensis extracts. The hexane, chloroform and aqueous extracts of root and bark of Q. borneensis were subjected to nitric oxide (NO) inhibition assay in LPS-stimulated RAW 264.7 cells. Expression of inducible NO synthase (iNOS) protein level was analyzed by Western blot. The antioxidant and antiproliferative activities of the extracts on HL-60 cells were determined using Ferric Reducing Antioxidant Power (FRAP) and MTT assays, respectively. The chloroform extract of Q. borneensis root obtained by soxhlet method (CSR) significantly inhibited 97.64 ± 0.96% of NO production (p < 0.001) and suppressed iNOS expression (p < 0.05) at the highest concentration of 1.0 μg/ml. The chloroform extract of bark obtained by maceration (CMB) exhibited the highest antioxidant capacity in the absence and presence of HL-60 cells, where the FRAP value were 125.45 ± 9.10 μM FeSO4.7H2O and 181.55 ± 3.45 μM FeSO4.7H2O, respectively. The greatest inhibition of HL-60 cell proliferation was exhibited by the chloroform extract of bark obtained by soxhlet method (CSB) with the IC50 of 5.0 μg/ml. The findings suggested that the chloroform extracts of Q. borneensis possess antiinflammatory, antioxidant and antiproliferative activities. |
| first_indexed | 2025-11-15T00:06:58Z |
| format | Article |
| id | oai:generic.eprints.org:11456 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:06:58Z |
| publishDate | 2017 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:114562018-03-14T10:01:40Z http://journalarticle.ukm.my/11456/ Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali, Quassia borneensis has been traditionally used as antihypertensive agent without any scientific literature on its mechanism of action. The objective of this study was to evaluate the antiinflammatory, antioxidant and antiproliferation properties of Q. borneensis extracts. The hexane, chloroform and aqueous extracts of root and bark of Q. borneensis were subjected to nitric oxide (NO) inhibition assay in LPS-stimulated RAW 264.7 cells. Expression of inducible NO synthase (iNOS) protein level was analyzed by Western blot. The antioxidant and antiproliferative activities of the extracts on HL-60 cells were determined using Ferric Reducing Antioxidant Power (FRAP) and MTT assays, respectively. The chloroform extract of Q. borneensis root obtained by soxhlet method (CSR) significantly inhibited 97.64 ± 0.96% of NO production (p < 0.001) and suppressed iNOS expression (p < 0.05) at the highest concentration of 1.0 μg/ml. The chloroform extract of bark obtained by maceration (CMB) exhibited the highest antioxidant capacity in the absence and presence of HL-60 cells, where the FRAP value were 125.45 ± 9.10 μM FeSO4.7H2O and 181.55 ± 3.45 μM FeSO4.7H2O, respectively. The greatest inhibition of HL-60 cell proliferation was exhibited by the chloroform extract of bark obtained by soxhlet method (CSB) with the IC50 of 5.0 μg/ml. The findings suggested that the chloroform extracts of Q. borneensis possess antiinflammatory, antioxidant and antiproliferative activities. Penerbit Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11456/1/16743-47128-1-SM.pdf Firdaus Kamarulzaman, and Julenah Ag Nuddin, and Kai, Li Lim and Aishah Adam, and Ahmad Sazali Hamzah, and Ahmad Rohi Ghazali, (2017) Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts. Jurnal Sains Kesihatan Malaysia, 15 (1). pp. 33-40. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/635 |
| spellingShingle | Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali, Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts |
| title | Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts |
| title_full | Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts |
| title_fullStr | Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts |
| title_full_unstemmed | Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts |
| title_short | Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts |
| title_sort | evaluation of antiinflammatory, antioxidant and antiproliferative activities of quassia borneensis noot. (simaroubaceae) extracts |
| url | http://journalarticle.ukm.my/11456/ http://journalarticle.ukm.my/11456/ http://journalarticle.ukm.my/11456/1/16743-47128-1-SM.pdf |