RNA extractions of mangosteen (Garcinia mangostana L.) pericarps for sequencing
This study employed several RNA extraction methods for mangosteen pericarps prior to RNA sequencing. The sequencing platform heavily relies on a high quality RNA yield. However, pericarp tissues contain a lot of phenolic compounds that results in low RNA quality. Hence, we studied several RNA extrac...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/11142/ http://journalarticle.ukm.my/11142/1/08%20Azhani%20Abdul%20Rahman.pdf |
| _version_ | 1848812624281600000 |
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| author | Azhani Abdul-Rahman, Najah Izzati Suleman, Wan Adibah Zakaria, Goh, Hoe-Han Normah Mohd Noor, Wan Mohd Aizat, |
| author_facet | Azhani Abdul-Rahman, Najah Izzati Suleman, Wan Adibah Zakaria, Goh, Hoe-Han Normah Mohd Noor, Wan Mohd Aizat, |
| author_sort | Azhani Abdul-Rahman, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | This study employed several RNA extraction methods for mangosteen pericarps prior to RNA sequencing. The sequencing platform heavily relies on a high quality RNA yield. However, pericarp tissues contain a lot of phenolic compounds that results in low RNA quality. Hence, we studied several RNA extraction methods to obtain the most suitable method for the best RNA quality from the pericarps of mangosteen. Five different methods including Lopez and Gomez, modified hexadecyltrimethyl ammonium bromide (CTAB) method, several commercial kits from TranszolUP, Favorgen and Qiagen RNeasy were compared. By optimising the CTAB method, it was found to be the best method to obtain pure RNA (high A260/A280 ratio) with the highest yields (up to approximately 600-800 ng/μL concentration). The QC control of these samples using bioanalyzer validated their suitability for the downstream RNA sequencing. This report details the method for extracting high quality and high yield RNA samples from fruit that are rich in polyphenolic compounds such as mangosteen. |
| first_indexed | 2025-11-15T00:05:15Z |
| format | Article |
| id | oai:generic.eprints.org:11142 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:05:15Z |
| publishDate | 2017 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:111422017-12-22T08:35:52Z http://journalarticle.ukm.my/11142/ RNA extractions of mangosteen (Garcinia mangostana L.) pericarps for sequencing Azhani Abdul-Rahman, Najah Izzati Suleman, Wan Adibah Zakaria, Goh, Hoe-Han Normah Mohd Noor, Wan Mohd Aizat, This study employed several RNA extraction methods for mangosteen pericarps prior to RNA sequencing. The sequencing platform heavily relies on a high quality RNA yield. However, pericarp tissues contain a lot of phenolic compounds that results in low RNA quality. Hence, we studied several RNA extraction methods to obtain the most suitable method for the best RNA quality from the pericarps of mangosteen. Five different methods including Lopez and Gomez, modified hexadecyltrimethyl ammonium bromide (CTAB) method, several commercial kits from TranszolUP, Favorgen and Qiagen RNeasy were compared. By optimising the CTAB method, it was found to be the best method to obtain pure RNA (high A260/A280 ratio) with the highest yields (up to approximately 600-800 ng/μL concentration). The QC control of these samples using bioanalyzer validated their suitability for the downstream RNA sequencing. This report details the method for extracting high quality and high yield RNA samples from fruit that are rich in polyphenolic compounds such as mangosteen. Penerbit Universiti Kebangsaan Malaysia 2017-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11142/1/08%20Azhani%20Abdul%20Rahman.pdf Azhani Abdul-Rahman, and Najah Izzati Suleman, and Wan Adibah Zakaria, and Goh, Hoe-Han and Normah Mohd Noor, and Wan Mohd Aizat, (2017) RNA extractions of mangosteen (Garcinia mangostana L.) pericarps for sequencing. Sains Malaysiana, 46 (8). pp. 1231-1240. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid46bil8_2017/KandunganJilid46Bil8_2017.html |
| spellingShingle | Azhani Abdul-Rahman, Najah Izzati Suleman, Wan Adibah Zakaria, Goh, Hoe-Han Normah Mohd Noor, Wan Mohd Aizat, RNA extractions of mangosteen (Garcinia mangostana L.) pericarps for sequencing |
| title | RNA extractions of mangosteen (Garcinia mangostana L.)
pericarps for sequencing |
| title_full | RNA extractions of mangosteen (Garcinia mangostana L.)
pericarps for sequencing |
| title_fullStr | RNA extractions of mangosteen (Garcinia mangostana L.)
pericarps for sequencing |
| title_full_unstemmed | RNA extractions of mangosteen (Garcinia mangostana L.)
pericarps for sequencing |
| title_short | RNA extractions of mangosteen (Garcinia mangostana L.)
pericarps for sequencing |
| title_sort | rna extractions of mangosteen (garcinia mangostana l.)
pericarps for sequencing |
| url | http://journalarticle.ukm.my/11142/ http://journalarticle.ukm.my/11142/ http://journalarticle.ukm.my/11142/1/08%20Azhani%20Abdul%20Rahman.pdf |